GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE36795 Query DataSets for GSE36795
Status Public on Feb 28, 2013
Title A role for the nucleoporin Nup170p in chromatin structure and gene silencing
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Genome binding/occupancy profiling by array
Expression profiling by array
Summary Embedded in the nuclear envelope, nuclear pore complexes (NPCs) not only regulate nuclear transport, but also interface with both transcriptionally active euchromatin and largely silenced heterochromatin, as well as the boundaries between these regions. It is unclear what functional role NPCs play in establishing or maintaining these distinct chromatin domains. Here we report that the yeast NPC protein Nup170p interacts with specific regions of the genome containing ribosomal protein and subtelomeric genes. At these locations, Nup170p functions to establish normal nucleosome positioning and as a repressor of transcription. We show that the function of Nup170p in subtelomeric gene silencing is linked to its association with the RSC chromatin-remodeling complex and the silencing factor Sir4p, and that the binding of Nup170p and Sir4p to subtelomeric chromatin is cooperative and necessary for the association of telomeres with the nuclear envelope. Our results establish the NPC as an active participant in the formation of peripheral heterochromatin.

This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series
Citation(s) 23452847
Submission date Mar 26, 2012
Last update date May 15, 2019
Contact name Yakun Wan
Organization name Southeast University
Street address Sipailou #2
City Nanjing
ZIP/Postal code 210096
Country China
Platforms (3)
GPL4131 Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A)
GPL6068 Agilent-011447 Yeast Oligo Microarray (G4140A) - alternative version
GPL9377 Illumina Genome Analyzer II (Saccharomyces cerevisiae)
Samples (14)
GSM901163 Genome-wide nucleosome positioning in wild type cells
GSM901164 Genome-wide nucleosome positioning in nup170∆ cells
GSM901165 nup157∆ gene expression analysis
This SuperSeries is composed of the following SubSeries:
GSE36792 The nucleoporin Nup170p is required for subtelomeric chromatin structure and gene silencing. [MNase-Seq]
GSE36793 A role for the nucleoporin Nup170p in chromatin structure and gene silencing [Agilent microarrays]
GSE36794 A role for the nucleoporin Nup170p in chromatin structure and gene silencing [Agilent ChIP-chip]
BioProject PRJNA157329

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36795_RAW.tar 420.1 Mb (http)(custom) TAR (of GFF, TSV, TXT)
SRA Run SelectorHelp

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap