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Series GSE36790 Query DataSets for GSE36790
Status Public on Dec 01, 2012
Title Cetuximab resistant or sensitive head and neck cancer samples
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
Summary 10 cell lines (five cetuximab sensitive and five cetuximab resistant) were selected for gene copy number array analysis on the Affymetrix SNP 6.0 platform. 39 protein coding genes were amplified in cetuximab resistant cells and normal in sensitive cells, all present on genomic regions 11q22.1 or 5p13-15. Five genes were selected for quantitative PCR verification, namely, YAP1 and TRPC6 (11q22.1) and PDCD6, TPPP, and PTGER4 (5p13-15). An extended panel of totally 10 cetuximab resistant and 10 sensitive cell lines verified that YAP1 amplified cells are cetuximab resistant. YAP1 gene amplification was highly correlated to the YAP1 mRNA expression, which was significantly higher in cetuximab resistant cells than in sensitive. YAP1 downregulation resulted in increased cetuximab sensitivity in one of two cetuximab resistant cell lines investigated and growth inhibition in another. We conclude that YAP1 is a marker for cetuximab resistance in head and neck cancer.
 
Overall design head and neck cancer cell lines with established cetuximab response were selected. 5 cetuximab resistant cell lines and 5 cetuximab sensitive cell lines were selected for gene genome wide gene copy number analysis on the Affymetrix SNP6.0 array
 
Contributor(s) Jerhammar F, Roberg K
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Submission date Mar 26, 2012
Last update date Nov 27, 2018
Contact name Fredrik Jerhammar
E-mail(s) fredrik.jerhammar@liu.se
Phone +4613221500
Organization name Linkoping University
Street address -
City Linkoping
ZIP/Postal code 58183
Country Sweden
 
Platforms (1)
GPL6801 [GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array
Samples (10)
GSM901092 UT-SCC-1A
GSM901093 UT-SCC-2
GSM901094 UT-SCC-16
Relations
BioProject PRJNA156745

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36790_RAW.tar 637.1 Mb (http)(custom) TAR (of CEL, CNCHP)
Processed data included within Sample table
Processed data provided as supplementary file

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