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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 23, 2019 |
| Title |
Influence of genetic variation on inducible gene expression in murine CD4+ splenocytes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Because activation of the immune response is dependent on extensive changes in gene expression, it is likely that a major component of inter-individual variation in the immune response is ultimately mediated at the level of gene regulation. Here, we examine the influence of genetic variation on inducible gene expression in the murine immune response. We extracted primary CD4 splenocytes from inbred strains A/J, C57BL/6J, BALB/c, DBA/2J, and 129x1/SvJ (3 animals/strain) and measured mRNA transcript levels using microarrays in both basal state and four hours after stimulation with PMA/Ionomycin.
We report strain-specific genome-wide differences in mRNA expression between A/J, Balb/c, C57BL/6J, DBA/2J and 129X1/SvJ inbred mice in CD4 splenocytes in basal, stimulated (4hr, PMA/I) states.
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| Overall design |
Splenocytes from A/J, Balb/c, C57BL/6J, DBA/2J and 129X1/SvJ inbred mouse strains were sorted for CD4 using autoMACS for RNA extraction and hybridization on Affymetrix microarrays. We examined the sorted cells at two time points; in the basal state (T0) and four hours after stimulation (T1) with a widely used, analogue of T cell receptor / CD28 co-stimulation signalling, namely phorbol 12-myristate 13-acetate (PMA) and ionomycin.
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| Web link |
https://openresearch-repository.anu.edu.au/handle/1885/156128
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| Contributor(s) |
French HJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 15, 2012 |
| Last update date |
Sep 23, 2019 |
| Contact name |
Hugh John French |
| E-mail(s) |
hughjohnfrench@gmail.com
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| Organization name |
UNSW
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| Street address |
UNSW
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| City |
Sydney |
| State/province |
New South Wales |
| ZIP/Postal code |
2036 |
| Country |
Australia |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (34)
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| GSM896768 |
A/J strain CD4 splenocyte at T0, biological rep1 |
| GSM896769 |
A/J strain CD4 splenocyte at T1, biological rep1 |
| GSM896770 |
A/J strain CD4 splenocyte at T0, biological rep2 |
| GSM896771 |
A/J strain CD4 splenocyte at T1, biological rep2 |
| GSM896772 |
A/J strain CD4 splenocyte at T0, biological rep4 |
| GSM896773 |
A/J strain CD4 splenocyte at T1, biological rep4 |
| GSM896774 |
A/J strain CD4 splenocyte at T0, biological rep5 |
| GSM896775 |
A/J strain CD4 splenocyte at T1, biological rep5 |
| GSM896776 |
Balb/c strain CD4 splenocyte at T0, biological rep1 |
| GSM896777 |
Balb/c strain CD4 splenocyte at T1, biological rep1 |
| GSM896778 |
Balb/c strain CD4 splenocyte at T0, biological rep2 |
| GSM896779 |
Balb/c strain CD4 splenocyte at T1, biological rep2 |
| GSM896780 |
Balb/c strain CD4 splenocyte at T0, biological rep4 |
| GSM896781 |
Balb/c strain CD4 splenocyte at T1, biological rep4 |
| GSM896782 |
C57BL/6J strain CD4 splenocyte at T0, biological rep1 |
| GSM896783 |
C57BL/6J strain CD4 splenocyte at T1, biological rep1 |
| GSM896784 |
C57BL/6J strain CD4 splenocyte at T0, biological rep2 |
| GSM896785 |
C57BL/6J strain CD4 splenocyte at T1, biological rep2 |
| GSM896786 |
C57BL/6J strain CD4 splenocyte at T0, biological rep3 |
| GSM896787 |
C57BL/6J strain CD4 splenocyte at T1, biological rep3 |
| GSM896788 |
C57BL/6J strain CD4 splenocyte at T0, biological rep4 |
| GSM896789 |
C57BL/6J strain CD4 splenocyte at T1, biological rep4 |
| GSM896790 |
DBA/2J strain CD4 splenocyte at T0, biological rep2 |
| GSM896791 |
DBA/2J strain CD4 splenocyte at T1, biological rep2 |
| GSM896792 |
DBA/2J strain CD4 splenocyte at T0, biological rep3 |
| GSM896793 |
DBA/2J strain CD4 splenocyte at T1, biological rep3 |
| GSM896794 |
DBA/2J strain CD4 splenocyte at T0, biological rep4 |
| GSM896795 |
DBA/2J strain CD4 splenocyte at T1, biological rep4 |
| GSM896796 |
129X1/SvJ CD4 splenocyte at T0, biological rep1 |
| GSM896797 |
129X1/SvJ CD4 splenocyte at T1, biological rep1 |
| GSM896798 |
129X1/SvJ CD4 splenocyte at T0, biological rep2 |
| GSM896799 |
129X1/SvJ strain CD4 splenocyte at T1, biological rep2 |
| GSM896800 |
129X1/SvJ strain CD4 splenocyte at T0, biological rep4 |
| GSM896801 |
129X1/SvJ strain CD4 splenocyte at T1, biological rep4 |
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| Relations |
| BioProject |
PRJNA153425 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36551_RAW.tar |
129.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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