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| Status |
Public on Sep 23, 2019 |
| Title |
Expression data from NOD and B10 CD4 splenocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Because activation of the immune response is dependent on extensive changes in gene expression, it is likely that a major component of inter-individual variation in the immune response is ultimately mediated at the level of gene regulation. Here, we examine the influence of genetic variation on inducible gene expression in the murine immune response.
We report strain-specific genome-wide differences in mRNA expression between NOD and B10 inbred mice in CD4 splenocytes in basal, stimulated (4hr, PMA/I) states.
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| Overall design |
Splenocytes from NOD and B10 inbred mouse strains were sorted for CD4 using autoMACS for RNA extraction and hybridization on Affymetrix microarrays. We examined the sorted cells at two time points; in the basal state (T0) and four hours after stimulation (T1) with a widely used, analogue of T cell receptor / CD28 co-stimulation signalling, namely phorbol 12-myristate 13-acetate (PMA) and ionomycin.
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| Web link |
https://openresearch-repository.anu.edu.au/handle/1885/156128
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| Contributor(s) |
French HJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 15, 2012 |
| Last update date |
Sep 23, 2019 |
| Contact name |
Hugh John French |
| E-mail(s) |
hughjohnfrench@gmail.com
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| Organization name |
UNSW
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| Street address |
UNSW
|
| City |
Sydney |
| State/province |
New South Wales |
| ZIP/Postal code |
2036 |
| Country |
Australia |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM896758 |
B10 strain CD4 splenocyte at T0, biological rep1 |
| GSM896759 |
B10 strain CD4 splenocyte at T0, biological rep2 |
| GSM896760 |
B10 strain CD4 splenocyte at T1, biological rep2 |
| GSM896761 |
B10 strain CD4 splenocyte at T0, biological rep3 |
| GSM896762 |
B10 strain CD4 splenocyte at T1, biological rep3 |
| GSM896763 |
NOD strain CD4 splenocyte at T0, biological rep1 |
| GSM896764 |
NOD strain CD4 splenocyte at T0, biological rep2 |
| GSM896765 |
NOD strain CD4 splenocyte at T1, biological rep2 |
| GSM896766 |
NOD strain CD4 splenocyte at T0, biological rep3 |
| GSM896767 |
NOD strain CD4 splenocyte at T1, biological rep3 |
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| Relations |
| BioProject |
PRJNA153423 |
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