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Series GSE36454 Query DataSets for GSE36454
Status Public on Jun 27, 2012
Title The CCR4-NOT Complex Is Implicated in the Viability of Aneuploid Yeasts
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by array
Summary To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period time and can form colonies by reducing the extent of the aneuploidy. We hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. Deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. We found that the CCR4-NOT complex, an evolutionarily conserved general regulator of mRNA turnover, and other related factors, including poly(A)-specific nuclease for mRNA decay, are involved in aneuploid viability. Defective mutations in CCR4-NOT complex components in the distantly related yeast Saccharomyces cerevisiae also affected the viability of spores produced from triploid cells, suggesting that this complex has a conserved role in aneuploids. In addition, our findings suggest that the genes required for homologous recombination repair are important for aneuploid viability.
 
Overall design Gene expression profile at exponentially-growing phase.in the fission yeast deletion mutants of the CCR4-NOT complex protein genes.
 
Contributor(s) Tange Y, Kurabayashi A, Goto B, Hoe K, Kim D, Park H, Hayles J, Chikashige Y, Tsutumi C, Hiraoka Y, Yamao F, Nurse P, Niwa O
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Submission date Mar 13, 2012
Last update date Jan 23, 2023
Contact name Atsushi Matsuda
Organization name National Institute of Information and Communications Technology
Street address 588-2, Iwaoka, Iwaoka-cho, Nishi-ku
City Kobe
ZIP/Postal code 651-2492
Country Japan
 
Platforms (1)
GPL15360 Agilent-028936 KARC-EX-2010-31 026058 [ProbeName version]
Samples (8)
GSM894045 S.pombe-wildtype-rep1
GSM894046 S.pombe-wildtype-rep2
GSM894047 S.pombe-not3D-rep1
Relations
BioProject PRJNA153549

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36454_RAW.tar 20.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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