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| Status |
Public on Jul 30, 2014 |
| Title |
Evaluation of Human Embryonic Stem Cells and their 1 Differentiated Fibroblastic Progenies as Cellular Models for In Vitro Genotoxicity Screening |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study evaluated human embryonic stem cells (hESC) and their differentiated fibroblastic progenies as cellular models for genotoxicity screening. The DNA damage response of hESCs and their differentiated fibroblastic progenies were compared to a fibroblastic cell line (HEPM, CRL1486) and primary cultures of peripheralblood lymphocytes (PBL), upon exposure to gamma irradiation.
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| Overall design |
Radiation exposure was carried out with Gamma ® 40 extractor with the central dose rate of 1.16 Gy/minute. Radiation dose 1 Gy was used in this study. Cells were harvested 30 minutes following radiation exposure. ESCs, H1F, HEPM and PBLs with and without 1 Gy radiation exposure were used for this purpose.
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| Contributor(s) |
MANIKANDAN J, VINOTH KJ, BALAKRISHNAN L, HENG A, LU K, SETHU S, HANDE MP, CAO T |
| Citation(s) |
24862194 |
| Submission date |
Mar 08, 2012 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Manikandan Jayapal |
| Organization name |
National University of Singapore
|
| Street address |
2 Medical Drive
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| City |
Singapore |
| ZIP/Postal code |
117597 |
| Country |
Singapore |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA153319 |
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