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Series GSE36094 Query DataSets for GSE36094
Status Public on Dec 20, 2012
Title Comparison of the gene expression profiles of a recombinant protein producing Hek 293 cell line and its non-producing parental cell line.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Comparison of the gene expression profiles of a recombinant protein producing Hek 293 cell line (referred to as producer) and its non-producing parental cell line Hek293F (referred to as non-producer). The parental cell line was obtained from Invitrogen, Carlsbad, CA. The producer was transfected with a heavy chain variable region fused to the Fc region of a human IgG (dAb-Fc). The aim of this study was to gain a better understanding of the process of recombinant protein production in Hek293 cells and to identify targets for the engineering of an improved host cell line.
 
Overall design Duplicate samples for RNA extraction were taken at four time points (approximately 30, 43, 51 and 67 hours after inoculation of the bioreactor) during the exponential phase of batch bioreactor cultures. Only one of these technical duplicates was analysed. Three bioreactor cultures were performed for each cell line representing three biological replicates resulting in 12 (3 x 4) samples per cell line.
 
Contributor(s) Dietmair S, Timmins NE, Nielsen LK
Citation(s) 22937046
Submission date Feb 27, 2012
Last update date Aug 13, 2018
Contact name Stefanie Dietmair
E-mail(s) stefanie.dietmair@googlemail.com
Organization name The University of Queensland
Department Australian Institute for Bioengineering and Nanotechnology
Street address Bldg 75
City Brisbane
State/province QLD
ZIP/Postal code 4072
Country Australia
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (24)
GSM881003 Producer culture - fermentation 1- time point 2
GSM881004 Producer culture - fermentation 2- time point 2
GSM881005 Producer culture - fermentation 3- time point 2
Relations
BioProject PRJNA151797

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36094_RAW.tar 26.2 Mb (http)(custom) TAR
GSE36094_non-normalized_data.txt.gz 4.4 Mb (ftp)(http) TXT
GSE36094_processed_data_filtered_by_detection-pvalue.txt.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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