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Series GSE36032 Query DataSets for GSE36032
Status Public on Apr 13, 2012
Title Activation of natural killer T cells promotes M2 macrophage polarization in adipose tissue and improves systemic glucose tolerance via the IL-4/STAT6 signaling axis in obesity
Organism Mus musculus
Experiment type Expression profiling by array
Summary Natural killer T (NKT) cells are important therapeutic targets in various disease models and under clinical trials for cancer patients. However, their function in obesity and type 2 diabetes remains unclear. Our data show that adipose tissues of both mice and humans contain a population of type-1 NKT cells, whose abundance decreases with increased adiposity and insulin resistance. Although loss-of-function of NKT cells had no effect on glucose tolerance in animals with prolonged high-fat diet (HFD) feeding, activation of NKT cells by lipid agonist α-galactosylceramide (αGalCer) enhances alternative macrophage polarization in adipose tissue and improves glucose homeostasis in animals at different stages of obesity. Furthermore, the effect of NKT cells is largely mediated by the IL-4/STAT6 signaling axis in obese adipose tissue. Thus, our data identifies a novel therapeutic target for the treatment of obesity-associated inflammation and type-2 diabetes.
Overall design Wild type and CD1d1 antigen (CD1d) knockout mice were fed a high fat diet for 4 days. On day 0 and 2 mice were injected intraperitoneally with α-galactosylceramide (αGalCer) or vehicle. On day 4 mice were killed and gene expression was profiled in epididymal adipose tissue.
Contributor(s) Ji Y, Sun S, Xu A, Yang L, Bhargava P, Lam KS, Gao B, Lee C, Kersten S, Qi L
Citation(s) 22396530
Submission date Feb 23, 2012
Last update date Apr 18, 2017
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (13)
GSM879464 WAT_WT_HFD_vehicle_repl1
GSM879465 WAT_WT_HFD_vehicle_repl2
GSM879466 WAT_WT_HFD_vehicle_repl3
BioProject PRJNA151749

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Supplementary file Size Download File type/resource
GSE36032_RAW.tar 59.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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