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Series GSE35940 Query DataSets for GSE35940
Status Public on Feb 20, 2012
Title A single N66S mutation in the PB1-F2 protein of influenza A virus increases virulence by inhibiting the early interferon response in vivo
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study used virological, histological, and global gene expression from an experimental murine model of influenza infection to study the contribution of a specific mutation in the PB1-F2 protein (PB1-F2 N66S) of influenza A to viral pathogenesis.
Overall design 6-8 week old, wild-type, female, C57Bl/6 mice were inoculated individually with 30 μl (10^4 PFU) of virus (recombinant influenza A/WSN/33 carrying the PB1 gene segment from A/Hong Kong/156/97 (H5N1) or a PB1 mutant recombinant virus resulting in an amino acid change at position 66 in the PB1-F2 protein [N66S]) in phosphate-buffered saline (PBS) containing penicillin-streptomycin and bovine serum albumin (PBS-BA-PS). A total of 10^4 PFU of virus was given in all inoculations. Control mice were given PBS-BA-PS. Lung samples were taken for microarray analysis at 12h, 1d, 3d, and 5d post-infection (n=3 animals per group at each time point for virus infected animals; n=2 animals per time point for mock-infected animals).
Contributor(s) Conenello GM, Tisoncik JR, Rosenzweig E, Varga ZT, Palese P, Katze MG
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Submission date Feb 20, 2012
Last update date Jan 12, 2017
Contact name Michael Katze
Organization name University of Washington
Department Microbiology
Lab Michael G. Katze, Ph.D
Street address Rosen Building 960 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109-4325
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (43)
GSM877804 lung_not infected (PBS mock)_M2_Day3_Exp2
GSM877805 lung_A/WSN/33 with PB1 from A/Hong Kong/156/97 (H5N1) containing N66S mutation_M1_Day1_Exp1
GSM877806 lung_A/WSN/33 with PB1 from A/Hong Kong/156/97 (H5N1)_M1_Day3_Exp1
BioProject PRJNA152087

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35940_RAW.tar 387.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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