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Series GSE35835 Query DataSets for GSE35835
Status Public on Oct 16, 2012
Title Small RNA profiles following TUT knock-down in HeLa
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The precise control of microRNA (miRNA) biogenesis is important for various cellular functions, and its dysregulation is often associated with human diseases. We previously reported that Terminal uridylyl transferase 4 (TUT4) down-regulates let-7 miRNA biogenesis by oligo-uridylating let-7 precursor (pre-let-7) in mouse embryonic stem cells and that a pluripotency marker Lin28 promotes a processivity of TUT4. Here we find that TUT4 positively controls let-7 biogenesis by adding a uridine residue to the 3’ end of pre-let-7 in the absence of Lin28. Such mono-uridylation enhances Dicer processing by generating an optimal end structure of pre-let-7 for Dicer recognition and may protect pre-miRNA from trimming. Moreover, TUT7, TUT4 and TUT2 redundantly regulate pre-let-7 processing and simultaneous knock down of these TUTs leads to the decrease of mature let-7 and the accumulation of pre-let-7 in cells. This study provides a novel regulation mechanism of miRNA biogenesis, which may function in development and tumorigenesis.
Overall design HeLa cells were transfected with siRNA two times over a 4~5 day period.
Contributor(s) Heo I, Ha M, Chang H, Kim VN
Citation(s) 23063654
Submission date Feb 15, 2012
Last update date May 15, 2019
Contact name Hyeshik Chang
Organization name Seoul National University
Department School of Biological Sciences
Lab Hyeshik Chang Lab
Street address Building 203 Room 525, School of Biological Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu
City Seoul
State/province South Korea
ZIP/Postal code 08826
Country South Korea
Platforms (2)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (5)
GSM876013 HeLa-siLuc-1
GSM876014 HeLa-siLuc-2
GSM876015 HeLa-siTUTs-1
This SubSeries is part of SuperSeries:
GSE40236 Small RNA and mRNA profiles following TUT knock-down in HeLa
SRA SRP010943
BioProject PRJNA151961

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