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Series GSE35775 Query DataSets for GSE35775
Status Public on May 30, 2012
Title The H3K27 demethylase Utx facilitates somatic and germ cell epigenetic reprogramming to pluripotency [Affymetrix gene expression]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Pluripotency can be induced in somatic cells by ectopic expression of defined transcription factors, however the identity of epigenetic regulators driving the progression of cellular reprogramming requires further investigation. Here we uncover a non-redundant role for the JmjC-domain-containing protein histone H3 methylated Lys 27 (H3K27) demethylase Utx, as a critical regulator for the induction, but not for the maintenance, of primed and naïve pluripotency in mice and in humans. Utx depletion results in aberrant H3K27me3 repressive chromatin demethylation dynamics, which subsequently hampers the reactivation of pluripotency promoting genes during reprogramming. Remarkably, Utx deficient primordial germ cells (PGCs) display a cell autonomous aberrant epigenetic reprogramming in vivo during their embryonic maturation, resulting in the lack of functional contribution to the germ-line lineage.
Overall design Samples include UTX+/Y (WT) and UTX-/Y (KO) cells from mouse ES cells and mouse embryonic fibroblast (MEF) before and after DOX induction (initiating reprogramming by OSKM factors). One sample is OSKM-induced Nanog-/- fibroblasts.
Contributor(s) Novershtern N, Gafni O
Citation(s) 22801502
Submission date Feb 13, 2012
Last update date Mar 04, 2019
Contact name Noa Novershtern
Organization name Weizmann Institute of Science
Department Molecular Genetics
Street address Weizmann Institute
City Rehovot
ZIP/Postal code 7610001
Country Israel
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (7)
GSM874650 V6.5 ESC Utx WT
GSM874651 V6.5 ESC Utx KO
GSM874652 V19-OSKM MEF Utx WT no DOX
This SubSeries is part of SuperSeries:
GSE37822 The H3K27 demethylase Utx facilitates somatic and germ cell epigenetic reprogramming to pluripotency
BioProject PRJNA152167

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35775_RAW.tar 29.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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