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Series GSE35733 Query DataSets for GSE35733
Status Public on May 10, 2012
Title Microarray analysis of gene expression differences in prostate specific antigen negative (PSA-ve) cells versus PSA+ve cells in human prostate cancer (HPCa) samples
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Prostate cancer (PCa) is heterogeneous containing both phenotypically differentiated and undifferentiated tumor cells. An important unanswered question is whether these two populations of PCa cells are functionally different. Here we report the distinct molecular, cellular, and tumor-propagating properties of PCa cells that express high (i.e., PSA+) and low (PSA-/lo) levels of the differentiation marker PSA (prostatespecific antigen). PSA-/lo PCa cells are quiescent and resistant to multiple stresses including androgen deprivation, exhibit high clonogenic potential, and possess long-term tumor-propagating capacity in male mice. They preferentially express stem cell-associated genes and can undergo asymmetric cell division generating PSA+ cells. Importantly, PSA-/lo PCa cells can initiate robust tumor development in castrated hosts, survive androgen deprivation, and harbor highly tumorigenic castration-resistant PCa cells that can be further enriched using the ALDH+CD44+α2β1+ phenotype. In contrast, PSA+ PCa cells possess more limited tumor-propagating capacity, mainly undergo symmetric division, and are sensitive to castration. Together, our study suggests that PSA-/lo and PSA+ PCa cells are functionally distinct and PSA-/lo cells may represent one critical source of castration-resistant PCa cells.
Overall design There are 4 sets of human samples in this study corresponding to 4 different prostate cancer patients labeled as HPCa46T, HPCa47T, HPCa49T and HPCa51T. Each set includes 3 technical triplicates (except HPCa46T) that were performed on dual color arrays and each individual array includes comparisons between PSA- (Cy5) and PSA+ (Cy3) cells of the respective patient prostate tumor.
Contributor(s) Qin J, Liu X, Laffin B, Chen X, Choy G, Jeter C, Calhoun-Davis T, Li H, Palapattu GS, Pang S, Lin K, Huang J, Ivanov I, Li W, Suraneni MV, Tang DG
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Submission date Feb 12, 2012
Last update date Feb 22, 2018
Contact name Dean G Tang
Organization name U.T M. D. Anderson Cancer Center
Department Carcinogenesis
Lab Dean G Tang's Lab
Street address 1808 Park road 1C
City Smithville
State/province TX
ZIP/Postal code 78957
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (10)
GSM874228 HPCa46T_PSA-ve/PSA+ve cells_rep1
GSM874229 HPCa47T_PSA-ve/PSA+ve cells_rep1
GSM874230 HPCa47T_PSA-ve/PSA+ve cells_rep2
This SubSeries is part of SuperSeries:
GSE35814 The PSA-/lo prostate cancer cells harbor self-renewing long-term tumor-propagating cells that resist castration
BioProject PRJNA155663

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35733_RAW.tar 149.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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