|
| Status |
Public on Mar 23, 2012 |
| Title |
High-throughput sequencing of endogenous small RNAs from rat vascular smooth muscle cells with and without Angiotensin II stimulation |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Angiotensin II (Ang II)-mediated vascular smooth muscle cells (VSMC) dysfunction plays a critical role in cardiovascular diseases. However, the role of microRNAs (miRNAs) in this process is unclear. We used small RNA deep sequencing to profile Ang II-regulated miRNAs in rat VSMC and evaluated their role in VSMC dysfunction. Sequencing results revealed several Ang II-responsive miRNAs and bioinformatics analysis showed that their predicted targets can modulate biological processes relevant to cardiovascular diseases.
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| |
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| Overall design |
Examined 4 samples of Rat VSMC. Control (without Ang II treatment) and 3 samples treated with Ang II for 1h, 3h, and 24h. Compared the changes in gene expression in Ang II treated samples relative to control samples.
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| |
|
| Contributor(s) |
Jin W, Reddy MA, Chen Z, Natarajan R |
| Citation(s) |
22431733 |
| |
| Submission date |
Feb 09, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Nancy Chen |
| E-mail(s) |
zhuchen@coh.org
|
| Organization name |
City of Hope
|
| Department |
Diabetes
|
| Street address |
1500 Duarte Road
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10669 |
Illumina Genome Analyzer IIx (Rattus norvegicus) |
|
| Samples (4)
|
| GSM873208 |
rat vascular smooth muscle cells after 48h serum depletion |
| GSM873209 |
rat vascular smooth muscle cells stimulated with Ang II for 1h |
| GSM873210 |
rat vascular smooth muscle cells stimulated with Ang II for 3h |
| GSM873211 |
rat vascular smooth muscle cells stimulated with Ang II for 24h |
|
| Relations |
| SRA |
SRP010854 |
| BioProject |
PRJNA152433 |
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