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Status |
Public on Feb 09, 2012 |
Title |
The invasion promoter Slug is a critical cell cycle regulator |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Background: The well-characterized function of the transcriptional repressor, Slug, is to promote EMT and tumor invasion/metastasis by down-regulating E-cadherin expression. In this study, we investigated the significance of Slug during the S phase. Method: Slug mRNA expression was isolated from thymidine-arrested CL1-5/AS2neo (control) and CL1-5/AS2neo-Slug-WT stable cells. The Agilent oligonucleotide microarray analysis was performed to identify Slug downstream genes. Results: Overexpression of Slug inhibited lung [3H]-thymidine incorporation and delayed S phase progression. By using Agilent microarray we have identified panel of genes altered by Slug overexpression. Slug can down-regulate target genes about cell cycle networks for DNA replication, DNA replication checkpoint and genomic stability, such as TOP1, ORC4, RFC3, and Rad17. Conclusions: the multifaceted role of Slug in cancer progression by controlling the epithelial-mesenchymal transition and genome stability.
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Overall design |
Two-condition experiment, Vector vs. Slug overexpression cells. The cDNAs encoding full-length human Slug were amplified and subcloned into lentiviral pLKO_AS2.neo which generated full-length Slug. Vector control or Slug lentivirus were transduced into CL1-5 cells and Gentamycin was used to select stable cells.
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Contributor(s) |
Wang WL, Hsu YC, Hong TM, Yang PC |
Citation missing |
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Submission date |
Feb 08, 2012 |
Last update date |
Nov 27, 2018 |
Contact name |
Tse-Ming Hong |
E-mail(s) |
htmjimmy@gmail.com
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Organization name |
Academia Sinica
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Street address |
128 Academia Road, Section 2, Nankang
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City |
Taipei |
ZIP/Postal code |
115 |
Country |
Taiwan |
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Platforms (1) |
GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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Samples (1) |
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Relations |
BioProject |
PRJNA152455 |