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Series GSE35617 Query DataSets for GSE35617
Status Public on Feb 09, 2012
Title The invasion promoter Slug is a critical cell cycle regulator
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: The well-characterized function of the transcriptional repressor, Slug, is to promote EMT and tumor invasion/metastasis by down-regulating E-cadherin expression. In this study, we investigated the significance of Slug during the S phase. Method: Slug mRNA expression was isolated from thymidine-arrested CL1-5/AS2neo (control) and CL1-5/AS2neo-Slug-WT stable cells. The Agilent oligonucleotide microarray analysis was performed to identify Slug downstream genes. Results: Overexpression of Slug inhibited lung [3H]-thymidine incorporation and delayed S phase progression. By using Agilent microarray we have identified panel of genes altered by Slug overexpression. Slug can down-regulate target genes about cell cycle networks for DNA replication, DNA replication checkpoint and genomic stability, such as TOP1, ORC4, RFC3, and Rad17. Conclusions: the multifaceted role of Slug in cancer progression by controlling the epithelial-mesenchymal transition and genome stability.
Overall design Two-condition experiment, Vector vs. Slug overexpression cells. The cDNAs encoding full-length human Slug were amplified and subcloned into lentiviral pLKO_AS2.neo which generated full-length Slug. Vector control or Slug lentivirus were transduced into CL1-5 cells and Gentamycin was used to select stable cells.
Contributor(s) Wang WL, Hsu YC, Hong TM, Yang PC
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Submission date Feb 08, 2012
Last update date Nov 27, 2018
Contact name Tse-Ming Hong
Organization name Academia Sinica
Street address 128 Academia Road, Section 2, Nankang
City Taipei
ZIP/Postal code 115
Country Taiwan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (1)
GSM871709 G1/S-synchronized CL1-5 cells
BioProject PRJNA152455

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Supplementary file Size Download File type/resource
GSE35617_RAW.tar 15.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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