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Series GSE35559 Query DataSets for GSE35559
Status Public on Feb 08, 2012
Title Gene expression profile induced by Imatinib
Organism Mus musculus
Experiment type Expression profiling by array
Summary Activation-induced cytidine deaminase (AID) is essential for class switch recombination (CSR) and somatic hypermutation (SHM). Its deregulated expression acts as a genomic mutator that can contribute to the development of various malignancies. During treatment with imatinib mesylate (IM), patients with chronic myeloid leukemia (CML) often develop hypogammaglobulinemia, the mechanism of which has not yet been clarified. Here, we provide evidence that CSR upon B cell activation is apparently inhibited by IM through downregulation of AID. Furthermore, expression of E2A, a key transcription factor for AID induction, was markedly suppressed by IM. These results elucidate not only the underlying mechanism of IM-induced hypogammaglobulinemia but also its potential efficacy as an AID suppressor.
Overall design We investigated that class switch recombination(CSR) upon B cell activation is apparently inhibited by imatinib mesylate(IM) through downregulation of activation-induced cytidine deaminase(AID). Furthermore, expression of E2A was markedly suppressed by IM. To elucidate the more detailed pathway, we performed the microarray analysis.
Microarray analysis was performed on splenocytes cultured for 72 h in the presence or absence of 10 µM Imatinib.
The mouse splenocytes were cultured for 72 h with or without 10 µM Imatinib (IM) in conditioning medium containing IL-4 and LPS. RNA from 1X10^6 splenocytes used for microarray analysis was isolated using the RNeasy Mini Kit (50) (Qiagen, Hilden, Germany). Gene expression microarray analysis was performed using one-color microarray-based gene-expression analysis (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions. After scanning, expression values for the genes were determined using GeneSpringGX software.
Contributor(s) Kawamata T, Jun L, Sato T, Tanaka M, Nagaoka H, Agata Y, Toyoshima T, Yokoyama K, Oyaizu N, Nakamura N, Ando K, Tojo A, Kotani A
Citation(s) 22337716
Submission date Feb 06, 2012
Last update date Nov 01, 2017
Contact name toyotaka kawamata
Organization name The Institute of Medical Science,The University of Tokyo
Department Hematology-Oncology/Molecular therapy
Street address 4-6-1,Shirokanedai,Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (4)
GSM870527 in vitro spleen cells 72hr IM(+)_rep1
GSM870528 in vitro spleen cells 72hr IM(+)_rep2
GSM870529 in vitro spleen cells 72hr IM(-)_rep1
BioProject PRJNA152679

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35559_RAW.tar 30.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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