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Series GSE35368 Query DataSets for GSE35368
Status Public on Mar 01, 2012
Title Deep-sequencing influences the results obtained in small-RNA sequencing
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary This study presents a comparison of small RNA sequencing libraries generated from the same cell lines but using different sequencing platforms and protocols. The samples were analyzed and compared at the level of miRNAs expression and as a population of small RNAs derived from repetitive elements. Despite a good correlation between sequencing platforms, there are qualitative and quantitative variations in the results depending on the protocol used.
Overall design 10 samples were examined: 6 from the ES E14 XY cell type: 1 454, 2 SOLiD from 2 technological versions, and 3 SOLEXA from 3 different protocols, and 4 samples from ES PGK XX cells: 1 454 and 1 SOLEXA sample, and 2 SOLiD samples from 2 technological versions.
Contributor(s) Toedling J, Servant N, Ciaudo C, Farinelli L, Voinnet O, Heard E, Barillot E
Citation(s) 22384282
Submission date Jan 26, 2012
Last update date May 15, 2019
Contact name Nicolas Servant
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platforms (5)
GPL9273 454 GS FLX (Mus musculus)
GPL9318 AB SOLiD System 3.0 (Mus musculus)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (10)
GSM866981 ES_XY_454
GSM866982 ES_XY_Solexa_Illu
GSM866983 ES_XY_Solexa_i_IDT
SRA SRP010603
BioProject PRJNA152595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35368_RAW.tar 513.6 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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