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Series GSE35361 Query DataSets for GSE35361
Status Public on May 01, 2012
Title JMJD3 is needed to regulate TGF beta responsive genes.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Neural development requires crosstalk between signaling pathways and chromatin. In this study, we demonstrate that neurogenesis is promoted by an interplay between the TGFβ pathway and the H3K27me3 histone demethylase (HDM) JMJD3. Genome-wide analysis showed that JMJD3 is targeted to gene promoters by Smad3 in neural stem cells (NSCs) and is essential to activate TGFβ-responsive genes. In vivo experiments in chick spinal cord revealed that the generation of neurons promoted by Smad3 is dependent on JMJD3 HDM activity. Overall, these findings indicate that JMJD3 function is required for the TGFβ developmental program to proceed
Overall design Mouse neural stem cells (NSC) control and JMJD3 knock down (shctrl and shJMJD3) were treated with the vehicle (-TGFb) or TGFbeta 5ng/ml (+TGFb) for 2.5h. Three replicates were performed for each point. And all of them were use it to posterior analysis.
Contributor(s) Estaras C, Martinez-Balbas M
Citation(s) 22782721
Submission date Jan 26, 2012
Last update date Jan 19, 2018
Contact name Conchi EI
Organization name IBMB
Street address Baldiri Reixac 15
City Barcelona
ZIP/Postal code 08028
Country Spain
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (12)
GSM866776 sh control - TGFb, replicate 1
GSM866777 sh control - TGFb, replicate 2
GSM866778 sh control - TGFb, replicate 3
BioProject PRJNA152629

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35361_RAW.tar 115.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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