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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 01, 2012 |
| Title |
JMJD3 is needed to regulate TGF beta responsive genes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Neural development requires crosstalk between signaling pathways and chromatin. In this study, we demonstrate that neurogenesis is promoted by an interplay between the TGFβ pathway and the H3K27me3 histone demethylase (HDM) JMJD3. Genome-wide analysis showed that JMJD3 is targeted to gene promoters by Smad3 in neural stem cells (NSCs) and is essential to activate TGFβ-responsive genes. In vivo experiments in chick spinal cord revealed that the generation of neurons promoted by Smad3 is dependent on JMJD3 HDM activity. Overall, these findings indicate that JMJD3 function is required for the TGFβ developmental program to proceed
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| Overall design |
Mouse neural stem cells (NSC) control and JMJD3 knock down (shctrl and shJMJD3) were treated with the vehicle (-TGFb) or TGFbeta 5ng/ml (+TGFb) for 2.5h. Three replicates were performed for each point. And all of them were use it to posterior analysis.
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| Contributor(s) |
Estaras C, Martinez-Balbas M |
| Citation(s) |
22782721 |
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| Submission date |
Jan 26, 2012 |
| Last update date |
Jan 19, 2018 |
| Contact name |
Conchi EI |
| Organization name |
IBMB
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| Street address |
Baldiri Reixac 15
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| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA152629 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35361_RAW.tar |
115.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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