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Series GSE35228 Query DataSets for GSE35228
Status Public on Jun 28, 2012
Title Cumulus cells genetic profile of patients stimulated with r-hLH or hCG evaluated with MicroArray analysis versus r-hFSH stimulated COCs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim of this study was to evaluate if different ovaric stimulation protocols modify gene expression profiles in COCs of infertile women. mRNA pools in COCs are essential for correct meiosis and activation of oocyte during fecondation and their alterations lead to reduced differentiation and oocyte functionality. In particular we tested the action of 2 different stimulation protocol on COCs transcriptome by MicroArray analysis of COCs after stimulation with r-hLH or h-CG versus COCs stimulated with r-hFSH. We identified genes and networks selectively altered after the different treatment protocols involved in proliferation and activity of COCs.
 
Overall design The study was based on MicroArray analysis of COCs obtained from infertile women after ovaric stimulation. We performed a total of 6 experiments using COCs pools from each woman. 4 experiments were biological replicates and 2 were dye swaps replicates.
 
Contributor(s) Gatta V, D'Aurora M, Franchi S, Sperduti S, Stuppia L, Tatone C, Artini P, Ciriminna R, Vento M
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Submission date Jan 20, 2012
Last update date Aug 28, 2014
Contact name Valentina Gatta
E-mail(s) v.gatta@unich.it
Phone 00390871541479
Fax 00390871541479
Organization name G. d’Annunzio University Foundation Chieti
Department Aging Research Center (CESI)
Lab Department of Medical Genetics
Street address Via Colle dell'Ara
City Chieti
State/province Abruzzo
ZIP/Postal code 66100
Country Italy
 
Platforms (1)
GPL6254 Phalanx Human OneArray
Samples (6)
GSM864186 hCG (hMG-HP)-treated COCs vs r-hFSH-treated COCs exp1
GSM864187 hCG (hMG-HP)-treated COCs vs r-hFSH-treated COCs exp2
GSM864188 r-hLH (r-hFSH+r-hLH)-treated COCs vs r-hFSH-treated COCs exp1
Relations
BioProject PRJNA152851

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35228_RAW.tar 17.7 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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