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Series GSE35125 Query DataSets for GSE35125
Status Public on Dec 21, 2013
Title Immunomagnetic purification of mES derived neuronal precursors increases neuronal migration and differentiation in vivo
Organism Mus musculus
Experiment type Expression profiling by array
Summary All established protocols for differentiation of mouse and human pluripotent stem cells into specific neural subpopulations generate a considerable cellular heterogeneity that hampers experimental and clinical progress. In order to obtain a homogenous population of neuronal precursor cells and to streamline the differentiation of embryonic stem cells (ESCs), we assessed PSA-NCAM, a surface glycoprotein that is specifically expressed on immature neurons. We developed an optimized strategy for magnetic isolation of PSA-NCAM positive neuronal precursors from differentiated ESC cultures and characterized their neuronal differentiation potential in vitro. PSA-NCAM enrichment at an early step of neural differentiation increased the number of ES cell derived neurons and reduced cellular diversity. Gene expression analysis revealed that mainly genes involved in neuronal activity were over-represented after purification. The in vivo potential of in vitro derived PSA-NCAM+ enriched precursors was functionally characterized by grafting into the forebrain of adult mice. Analysis for several neuronal and glia markers at 10 or 40 days post graft showed a distinct differentiation pattern. While unsorted control cells gave rise to a mixed population composed of immature precursors, early postmitotic neurons or glial cells, the majority of PSA-NCAM+ enriched cells differentiated into NeuN positive neurons. Furthermore, when in contact with the rostral migratory stream, higher numbers of cells integrated into the stream and migrated towards the olfactory bulb when the PSA-NCAM enriched population was grafted. Thus, enrichment of neuronal precursors based on PSA-NCAM expression represents a general and straightforward approach to narrow cellular heterogeneity during neuronal differentiation of pluripotent cells.
Overall design Two conditions (step 4, step 5), each represented by three biological replicates of control and enriched cells (Cy5); mESC was used as common reference (Cy3)
Contributor(s) Barral S, Ecklebe J, Tomiuk S, Tiveron M, Desoeuvre A, Eckardt D, Cremer H, Bosio A
Citation(s) 23237958
Submission date Jan 16, 2012
Last update date Nov 01, 2017
Contact name Stefan Tomiuk
Organization name Miltenyi Biotec GmbH
Department Bioinformatics
Street address Friedrich-Ebert-Str. 68
City Bergisch-Gladbach
ZIP/Postal code 51429
Country Germany
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (12)
GSM862323 neural-precursors_no-enrichment_rep1
GSM862324 neural-precursors_no-enrichment_rep2
GSM862325 neural-precursors_no-enrichment_rep3
BioProject PRJNA150721

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Supplementary file Size Download File type/resource
GSE35125_RAW.tar 185.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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