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Series GSE34929 Query DataSets for GSE34929
Status Public on Jun 28, 2012
Title Gene expression profile of Wharton-jelly mesenchymal stem cells at 12th in vitro expansion cycle compared to Wharton-jelly mesenchymal stem cells at 4th in vitro expansion cycle
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Mesenchymal stem cells (MSCs) are generally considered the main tool box for cell-based therapies and compared to embryonic stem cells (ESCs), MSCs exhibit many advantages. It has been shown that UC matrix (the Wharton’s Jelly surrounding umbilical vessels) contains a great number of mesenchymal cells. The abundant amount of Wharton’s Jelly (WJ) makes it an attractive source of MSCs for cell-based therapies. recently have been highlighted the changes in protein expression profiling during in vitro expansion of WJ-MSC, probably related to the gradual reduction of their staminal plasticity and to the biological cellular mechanism occurring in the cellular aging. The aim of the present work was to analyze the transcriptomic profile useful to the definition of WJ-MSC characteristics and therefore identify possible different new marker candidates. In these contest, changes in gene expression profiles occurring during WJ-MSC in vitro growth wile by analyzed in order to find possible modulation related to their long expansion and fast growth abilities. We analyzed the changes in total RNA expression at the 12th in vitro expansion cycle compared to the 4th in vitro expansion cycle.
Overall design In this study we performed gene expression profiles of a total of 6 experiment. Three different cell pools at 12th and 4th in vitro expansion cycles were used (UC2, UCA and UCB). On the total of 6 experiments 3 were biological replicates, 2 technical replicates and 1 dye-swap replicate.
Contributor(s) Gatta V, D'Aurora M, Franchi S, Marchisio M, Pierdomenico L, Lanuti P, Miscia S, Stuppia L, Palka G
Citation(s) 24053474
Submission date Jan 09, 2012
Last update date Aug 22, 2019
Contact name Valentina Gatta
Phone 00390871541479
Fax 00390871541479
Organization name G. d’Annunzio University Foundation Chieti
Department Aging Research Center (CESI)
Lab Department of Medical Genetics
Street address Via Colle dell'Ara
City Chieti
State/province Abruzzo
ZIP/Postal code 66100
Country Italy
Platforms (1)
GPL6254 Phalanx Human OneArray
Samples (6)
GSM831050 WJ-MSC 12vs4 exp1
GSM856028 WJ-MSC 12vs4 exp2
GSM856029 WJ-MSC 12vs4 exp3
BioProject PRJNA150997

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Supplementary file Size Download File type/resource
GSE34929_RAW.tar 17.9 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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