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Series GSE34916 Query DataSets for GSE34916
Status Public on Aug 01, 2015
Title Expression data from cyclohexamide treated Human Foreskin Fibroblasts (HFFs) infected with type I Toxoplasma gondii and stimulated with interferon gamma.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Pre-infection with Toxoplasma parasites inhibits induction of an interferon gamma responsive gene expression program. In this study we aimed to determine what role host negative regulatory proteins that are induced by Toxoplasma infection, such as SOCS-1, have in this inhibition.
We used Affymetrix microarrays to analyze host cell transcription after pre-treatment with the protein synthesis inhibitor cyclohexamide (CHX), Toxoplasma infection, and subsequent interferon-gamma stimulation.
Overall design Human foreskin fibroblasts (HFFs) were left untreated or treated with CHX for 40min. Cells were infected, or not, with type I (RH) Toxoplasma parasites, for one hour, and subsequently stimulated with human IFN-γ for one hour. Total RNA was isolated and hybridized to Affymetrix Human U133A 2.0 gene chips.
Contributor(s) Saeij JP, Rosowski EE
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Submission date Jan 06, 2012
Last update date Dec 06, 2018
Contact name Emily E Rosowski
Organization name MIT
Department Biology
Lab Saeij
Street address 77 Massachusetts Ave, 68-258d
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM857488 HFF-untreat,uninfect,unstim
GSM857489 HFF-untreat,uninfect,IFNY
GSM857490 HFF-CHX,uninfect,unstim
BioProject PRJNA150163

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34916_RAW.tar 17.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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