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Series GSE34903 Query DataSets for GSE34903
Status Public on Apr 01, 2012
Title Gene expression profiles of tumor-associated macrophages (TAMs) overexpressing miR-511-3p
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hematopoietic stem/progenitor cells (HS/PCs) were transduced with lentiviral vectors overexpressing OFP and either miR-511-3p or a control, mutated miRNA sequence (miR-511-3p-mut). The transduced HS/PCs were then transplanted in recipient C57BL/6 mice. Tumors (Lewis lung carcinomas, LLC) were injected s.c. 4 weeks after HS/PC transplant. Lentiviral vector-transduced (OFP+), tumor-associated macrophages (TAMs) were isolated 4 weeks after LLC injection by fluorescence-activated cell sorting. Gene expression profiles of TAMs overexpressing either miR-511-3p or miR-511-3p-mut were obtained from 3 independent biological samples/each. Gene expression profiles of miRNA-overexpressing TAMs were then compared with the gene expression profiles of wild-type TAMs isolated from LLCs grown in nontransplanted C57BL/6 mice; the latter TAMs were subfractioned into MRC1+CD11c(low) or CD11c+MRC1(low) subsets before RNA isolation and analysis. Comparison of gene expression profiles of TAMs revealed that miR-511-3p overexpression tunes down the expression of multiple genes that are classically upregulated in protumoral MRC1+CD11c(low) TAMs.
Overall design TAMs were isolated from Lewis lung carcinomas grown s.c. in C57BL/6 mice.
Contributor(s) Squadrito ML, De Palma M, Lyle R, Gilfillan GD
Citation(s) 22832163
Submission date Jan 06, 2012
Last update date May 15, 2019
Contact name Camilla Stormo
Organization name Oslo University Hospital
Department Department of Medical Genetics
Street address Kirkeveien 166
City Oslo
ZIP/Postal code 0407
Country Norway
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (12)
GSM857324 MRC1+TAMs_001
GSM857325 MRC1+TAMs_002
GSM857326 MRC1+TAMs_003
SRA SRP010190
BioProject PRJNA150173

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Supplementary file Size Download File type/resource
GSE34903_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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