GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE34854 Query DataSets for GSE34854
Status Public on Feb 17, 2012
Title Transcriptome of double-positive thymocyte precursors transduced with a retrovirus encoding Lin28a
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed paired-end RNA-seq to compare the transcriptome of DP thymocytes that ectopically express Lin28 in vivo versus untransduced (GFP-ve) DP thymocytes.
Overall design Transduced (GFP+) and untransduced (GFP-) CD4+ CD8+ CD3- thymocytes were sorted and pooled from three recipients of hematopoietic stem and progenitor cells transduced with Lin28-RV six weeks post-reconstitution. Total RNA was extracted and paired-end library construction and sequencing was performed on oligo-dT purified RNA.
Contributor(s) Yuan J, Burr P, Nguyen CK, Muljo SA
Citation(s) 22345399
Submission date Jan 04, 2012
Last update date May 15, 2019
Contact name Cuong Kieu Nguyen
Phone 301-451-3227
Organization name NIH
Department NIAID
Lab Laboratory of Immunology
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM856150 Lin28-RV GFP+ DP thymocytes
GSM856151 Lin28-RV GFP- DP thymocytes
SRA SRP010106
BioProject PRJNA150133

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34854_RAW.tar 545.8 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap