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| Status |
Public on Feb 17, 2012 |
| Title |
Transcriptome of double-positive thymocyte precursors transduced with a retrovirus encoding Lin28a |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed paired-end RNA-seq to compare the transcriptome of DP thymocytes that ectopically express Lin28 in vivo versus untransduced (GFP-ve) DP thymocytes.
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| Overall design |
Transduced (GFP+) and untransduced (GFP-) CD4+ CD8+ CD3- thymocytes were sorted and pooled from three recipients of hematopoietic stem and progenitor cells transduced with Lin28-RV six weeks post-reconstitution. Total RNA was extracted and paired-end library construction and sequencing was performed on oligo-dT purified RNA.
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| Contributor(s) |
Yuan J, Burr P, Nguyen CK, Muljo SA |
| Citation(s) |
22345399 |
| Submission date |
Jan 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Cuong Kieu Nguyen |
| E-mail(s) |
nguyenck@mail.nih.gov
|
| Phone |
301-451-3227
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
Laboratory of Immunology
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
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| Relations |
| SRA |
SRP010106 |
| BioProject |
PRJNA150133 |
