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| Status |
Public on Nov 07, 2005 |
| Title |
Identification of LEDGF-regulated genes. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The LEDGF transcript from the PSIP1 gene was knocked down using RNAi technology. The resulting 293T derived cell line (293TsiLL) was compared to a control cell line (293TsiScram) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration.
Keywords: gene knockdown effects
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| |
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| Overall design |
2 cell lines were used, 293TsiLL (experimental) and 293TsiScram (control). 4 independent cultures of each cell line were used for independent RNA extractions, labeling reactions and array hybridizations.
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| Contributor(s) |
Ciuffi A, Llano M, Poeshla E, Hoffmann C, Leipzig J, Shinn P, Ecker J, Bushman FD |
| Citation(s) |
16311605 |
| Submission date |
Oct 20, 2005 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Chris Hoffmann |
| Organization name |
University of Pennsylvania
|
| Street address |
3610 hamilton walk
|
| City |
philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA93303 |
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