GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE34818 Query DataSets for GSE34818
Status Public on Feb 01, 2013
Title MiR-199a-5p determines fibroblast activation and pulmonary fibrogenesis
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary Idiopathic pulmonary fibrosis (IPF) is a chronic and often fatal pulmonary disorder characterized by fibroblast proliferation and the excess deposit of extracellular matrix proteins. The etiology of IPF is unknown, but a central role for microRNAs (miRNAs), a class of small non-coding regulatory RNAs, has been recently suggested. We report the upregulation of miR-199a-5p in mouse lungs undergoing bleomycin-induced fibrosis and also in human biopsies from IPF patients. Levels of miR-199a-5p were increased selectively in myofibroblasts and putative profibrotic effects of miR-199a-5p were further investigated in cultured lung fibroblasts. MiR-199a-5p expression was induced upon TGFβ exposure and ectopic expression of miR-199a-5p was sufficient to promote the pathogenic activation of pulmonary fibroblasts. CAV1, a critical mediator of pulmonary fibrosis, was established as a bona fide target of miR-199a-5p. Finally, we also found an aberrant expression of miR-199a-5p in mouse models of kidney and liver fibrosis, suggesting that dysregulation of miR-199a-5p represents a general mechanism contributing to the fibrotic process. We propose miR-199a-5p as a major regulator of fibrosis that represents a potential therapeutic target to treat fibroproliferative diseases.

This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series
Contributor(s) Mari B, Pottier N, Barbry P, Lebrigand K, Hénaoui I
Citation(s) 23459460
Submission date Jan 03, 2012
Last update date Nov 27, 2018
Contact name Kevin Lebrigand
Organization name IPMC/CNRS
Lab Functional Genomics Platform of Nice-Sophia-Antipolis, France.
Street address 660 route des lucioles
City Valbonne - Sophia-Antipolis
ZIP/Postal code 06560
Country France
Platforms (3)
GPL7686 IPMC - microRNA v7
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (48)
GSM855718 353051-2
GSM855719 353051-1
GSM855720 353232-2
This SuperSeries is composed of the following SubSeries:
GSE34812 miRNA reponse to bleomycin instillation
GSE34814 mRNA reponse to bleomycin instillation
GSE34815 Impact of miR-199-5p overexpression and CAV1 silencing on human lung fibroblasts
BioProject PRJNA150247

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34818_RAW.tar 309.2 Mb (http)(custom) TAR (of GPR, TXT)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap