|Public on Feb 01, 2013
|MiR-199a-5p determines fibroblast activation and pulmonary fibrogenesis
|Homo sapiens; Mus musculus
|Expression profiling by array
Non-coding RNA profiling by array
|Idiopathic pulmonary fibrosis (IPF) is a chronic and often fatal pulmonary disorder characterized by fibroblast proliferation and the excess deposit of extracellular matrix proteins. The etiology of IPF is unknown, but a central role for microRNAs (miRNAs), a class of small non-coding regulatory RNAs, has been recently suggested. We report the upregulation of miR-199a-5p in mouse lungs undergoing bleomycin-induced fibrosis and also in human biopsies from IPF patients. Levels of miR-199a-5p were increased selectively in myofibroblasts and putative profibrotic effects of miR-199a-5p were further investigated in cultured lung fibroblasts. MiR-199a-5p expression was induced upon TGFβ exposure and ectopic expression of miR-199a-5p was sufficient to promote the pathogenic activation of pulmonary fibroblasts. CAV1, a critical mediator of pulmonary fibrosis, was established as a bona fide target of miR-199a-5p. Finally, we also found an aberrant expression of miR-199a-5p in mouse models of kidney and liver fibrosis, suggesting that dysregulation of miR-199a-5p represents a general mechanism contributing to the fibrotic process. We propose miR-199a-5p as a major regulator of fibrosis that represents a potential therapeutic target to treat fibroproliferative diseases.
This SuperSeries is composed of the SubSeries listed below.
|Refer to individual Series
|Mari B, Pottier N, Barbry P, Lebrigand K, Hénaoui I
|Jan 03, 2012
|Last update date
|Nov 27, 2018
|Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|660 route des lucioles
|Valbonne - Sophia-Antipolis
|IPMC - microRNA v7
|Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
|Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
|This SuperSeries is composed of the following SubSeries:
|miRNA reponse to bleomycin instillation
|mRNA reponse to bleomycin instillation
|Impact of miR-199-5p overexpression and CAV1 silencing on human lung fibroblasts