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Series GSE34815 Query DataSets for GSE34815
Status Public on Feb 01, 2013
Title Impact of miR-199-5p overexpression and CAV1 silencing on human lung fibroblasts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify putative novel specific targets of mir-199-5p, we overexpressed miR-199a-5p as well as miR-21 and a siRNA targeted against CAV1 in human HFL1 pulmonary fibroblasts (CCL-153) by transfecting them with synthetic pre-miRNAs or a synthetic “negative” pre-miRNA as control (miR-Neg). RNA samples were harvested at 48 hours post-transfection and 2 independent experiments were carried out. Additional samples correspond to HFL1 cells treated or not with 10ng/ml TGFbeta for 48 hours in the absence of serum (2 independent experiments).
Overall design 2 independent experiments performed in a one color design, corresponding to 7 conditions (miR-Neg, miR-199-5p, miR-21, si-Neg, si-CAV1, control, TGFbeta) and a total of 14 samples.
Contributor(s) Pottier N, Mari B, Barbry P, Lebrigand K
Citation(s) 23459460
Submission date Jan 03, 2012
Last update date Nov 27, 2018
Contact name Kevin Lebrigand
Organization name IPMC/CNRS
Lab Functional Genomics Platform of Nice-Sophia-Antipolis, France.
Street address 660 route des lucioles
City Valbonne - Sophia-Antipolis
ZIP/Postal code 06560
Country France
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (14)
GSM855753 252800411748-7
GSM855754 252800411748-6
GSM855755 252800411748-5
This SubSeries is part of SuperSeries:
GSE34818 MiR-199a-5p determines fibroblast activation and pulmonary fibrogenesis
BioProject PRJNA156287

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34815_RAW.tar 175.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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