|
| Status |
Public on Feb 01, 2013 |
| Title |
Impact of miR-199-5p overexpression and CAV1 silencing on human lung fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify putative novel specific targets of mir-199-5p, we overexpressed miR-199a-5p as well as miR-21 and a siRNA targeted against CAV1 in human HFL1 pulmonary fibroblasts (CCL-153) by transfecting them with synthetic pre-miRNAs or a synthetic “negative” pre-miRNA as control (miR-Neg). RNA samples were harvested at 48 hours post-transfection and 2 independent experiments were carried out. Additional samples correspond to HFL1 cells treated or not with 10ng/ml TGFbeta for 48 hours in the absence of serum (2 independent experiments).
|
| |
|
| Overall design |
2 independent experiments performed in a one color design, corresponding to 7 conditions (miR-Neg, miR-199-5p, miR-21, si-Neg, si-CAV1, control, TGFbeta) and a total of 14 samples.
|
| |
|
| Contributor(s) |
Pottier N, Mari B, Barbry P, Lebrigand K |
| Citation(s) |
23459460 |
| Submission date |
Jan 03, 2012 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (14)
|
|
| This SubSeries is part of SuperSeries: |
| GSE34818 |
MiR-199a-5p determines fibroblast activation and pulmonary fibrogenesis |
|
| Relations |
| BioProject |
PRJNA156287 |
Less...
More...