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| Status |
Public on Dec 22, 2011 |
| Title |
Methylation data from CD4+ cells at birth and 12 months from a cohort of children who were diagnosed with IgE food allergy to either egg milk or peanut at 12 months (n=12) and healthy controls (n=12). |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
DNA methylation data derived from total CD4+ T-cells from a cohort of paediatric food allergy. There are 12 food allergic samples (6 activated and matched control), 12 non-allergic samples (6 activated and matched control) collected at birth and resample at 12-months, total 48 samples.
Mononuclear cells were either activated with anti-CD3 (0.5 ug/mL) + IL2(20U) or rested in media alone for 24 hours. CD4+ cells were purified and DNA was harvested and bisulphite converted for methylation analysis on illumina HM450K array.
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| Overall design |
This is a pooled design. Each sample is bisulphite converted gDNA pooled in equimolar amounts from n=2 individuals. There are 6 allergic pools and 6 non allergic pools. Each pool is sampled at two time points, birth and 12 months, and either treated or untreated at each time point.
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| Citation(s) |
22495533, 24762976 |
| Submission date |
Dec 21, 2011 |
| Last update date |
Mar 22, 2019 |
| Contact name |
David Martino |
| E-mail(s) |
david.martino@telethonkids.org.au
|
| Phone |
0863191635
|
| Organization name |
Telethon Kids Institute
|
| Street address |
Perth Childrens Hospital, 15 Hospital Avenue
|
| City |
NEDLANDS |
| State/province |
Western Australia |
| ZIP/Postal code |
6009 |
| Country |
Australia |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA151263 |
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