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Series GSE34639 Query DataSets for GSE34639
Status Public on Dec 22, 2011
Title Methylation data from CD4+ cells at birth and 12 months from a cohort of children who were diagnosed with IgE food allergy to either egg milk or peanut at 12 months (n=12) and healthy controls (n=12).
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary DNA methylation data derived from total CD4+ T-cells from a cohort of paediatric food allergy. There are 12 food allergic samples (6 activated and matched control), 12 non-allergic samples (6 activated and matched control) collected at birth and resample at 12-months, total 48 samples.
Mononuclear cells were either activated with anti-CD3 (0.5 ug/mL) + IL2(20U) or rested in media alone for 24 hours. CD4+ cells were purified and DNA was harvested and bisulphite converted for methylation analysis on illumina HM450K array.
Overall design This is a pooled design. Each sample is bisulphite converted gDNA pooled in equimolar amounts from n=2 individuals. There are 6 allergic pools and 6 non allergic pools. Each pool is sampled at two time points, birth and 12 months, and either treated or untreated at each time point.
Citation(s) 22495533, 24762976
Submission date Dec 21, 2011
Last update date Mar 22, 2019
Contact name David Martino
Phone 0863191635
Organization name Telethon Kids Institute
Street address Perth Childrens Hospital, 15 Hospital Avenue
State/province Western Australia
ZIP/Postal code 6009
Country Australia
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (48)
GSM852282 FA_CD3__CB_1
GSM852283 FA_CD3__CB_2
GSM852284 FA_CD3__CB_3
BioProject PRJNA151263

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34639_RAW.tar 183.1 Mb (http)(custom) TAR
GSE34639_matrix_signal_intensities.txt.gz 118.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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