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Series GSE34546 Query DataSets for GSE34546
Status Public on Apr 24, 2013
Title Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Background.
The histone variant H3.3 plays key roles in regulating chromatin states and transcription. However, the role of endogenous H3.3 in mammalian cells and during development has been less thoroughly investigated. To address this gap, we report the production and phenotypic analysis of mice and cells with targeted disruption of the H3.3-encoding gene, H3f3b.
H3f3b KO mice exhibit a semi-lethal phenotype traceable at least in part to defective cell division and chromosome segregation. H3f3b KO cells have widespread ectopic CENP-A protein localization suggesting one possible mechanism for defective chromosome segregation. KO cells have abnormal karyotypes and cell cycle profiles as well. The transcriptome and euchromatin-related epigenome were moderately affected by loss of H3f3b in MEFs with ontology most notably pointing to changes in chromatin regulatory and histone coding genes. Reduced numbers of H3f3b KO mice survive to maturity and almost all survivors from both sexes are infertile.
Taken together, our studies suggest that endogenous mammalian histone H3.3 has important roles in regulating chromatin and chromosome functions that in turn are important for cell division, genome integrity, and development.
Overall design Examination of H3K9Ac and H3K4me3 in wild-type and H3.3 null MEFs
Contributor(s) O'Geen H, Bush KM, Yuen BT, Barrilleaux BL, Riggs JW, Cotterman R, Knoepfler PS
Citation(s) 23570311
Submission date Dec 19, 2011
Last update date May 15, 2019
Contact name Henriette O'Geen
Organization name UC Davis
Street address 1 Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM863801 TMK4_MEF2_KO
GSM863802 TMK4_MEF3_WT
GSM863803 TMK4_MEF7_KO
SRA SRP010408
BioProject PRJNA151369

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Supplementary file Size Download File type/resource
GSE34546_RAW.tar 824.2 Mb (http)(custom) TAR (of BED, GFF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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