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Series GSE34486 Query DataSets for GSE34486
Status Public on Apr 10, 2012
Title DNA methylation regulates lineage-specifying genes in the human vascular system [methylation array].
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary During embryonic development, the lymphatic system emerges by transdifferentiation from the cardinal vein. Although lymphatic and blood vasculature share a close molecular and developmental relationship, they display distinct features and functions. However, even after terminal differentiation, transitions between the two endothelial cell types have been reported. Since changes in phenotypic plasticity and cellular differentiation processes frequently involve epigenetic mechanisms, we wondered whether DNA methylation might play a role in regulating cell type-specific expression in endothelial cells. By analyzing global gene expression and methylation patterns of primary human dermal lymphatic and blood endothelial cells, we identified a highly significant set of genes, which were differentially methylated and expressed. Pathway analyses of the differentially methylated and upregulated genes in lymphatic endothelial cells revealed involvement in developmental and transdifferentiation processes. We further identified a set of novel genes, which might be implicated in regulating BEC-LEC plasticity and could serve as therapeutic targets and/or biomarkers in vascular diseases associated with alterations in the endothelial phenotype.
Overall design Bisulphite converted DNA from the 16 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip v1.1
Contributor(s) Broenneke S, Brueckner B, Peters N, Bosch TC, Stäb F, Wenck H, Hagemann S, Winnefeld M
Citation(s) 22434260
Submission date Dec 16, 2011
Last update date Mar 22, 2019
Contact name Silvia Rueberg
Organization name Miltenyi Biotec GmbH
Department Genomic Services Department
Street address Friedrich-Ebert-Str. 68
City Bergisch Gladbach
ZIP/Postal code 51429
Country Germany
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (16)
GSM849974 HDLEC_5112.L
GSM849975 HDBEC_60w.Br.B
GSM849976 HDLEC_3222.L
This SubSeries is part of SuperSeries:
GSE34487 DNA methylation regulates lineage-specifying genes in the human vascular system.
BioProject PRJNA156341

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34486_RAW.tar 183.1 Mb (http)(custom) TAR
GSE34486_non_normalized.txt.gz 67.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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