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Series GSE34280 Query DataSets for GSE34280
Status Public on Dec 09, 2011
Title Clonal Selection Drives Genetic Divergence of Metastatic Medulloblastoma [Affymetrix SNP6 Arrays]
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
Summary Medulloblastoma, the most common malignant pediatric brain tumour, disseminates by shedding cells into the cerebrospinal fluid, which then re-implant to cover the surface of the brain and spinal cord. Metastases are a very poor prognostic sign at presentation and are usually lethal at recurrence. Mechanisms driving dissemination have been described in the bulk primary tumour, with the underlying assumption that primary tumour and metastases are biologically similar. Here we show that in both mouse and human medulloblastoma, multiple metastases from a single animal are extremely similar, but are genetically highly divergent from the primary tumour. Clonal genetic events in the metastases can be demonstrated in a restricted sub-clone of the primary tumour, suggesting that only rare cells within the primary tumour have the ability to metastasize. Failure to account for the bicompartmental nature of primary and metastatic medulloblastoma represents a major barrier to the development of effective targeted therapies.
Overall design Affymetrix SNP arrays were performed according to the manufacturer's directions on DNA extracted from cryopreserved human medulloblastoma tissue samples.
Copy number analysis of Affymetrix SNP6 arrays was performed for 17 pediatric medulloblastoma samples. Samples comprise a series of 7 patient-matched primary/metastatic cases.
Contributor(s) Northcott PA, Wu X, Taylor MD
Citation(s) 22343890
Submission date Dec 08, 2011
Last update date Nov 27, 2018
Contact name Paul A. Northcott
Organization name German Cancer Research Center (DKFZ)
Street address Im Neuenheimer Feld 280
ZIP/Postal code 69120
Country Germany
Platforms (1)
GPL6801 [GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array
Samples (17)
GSM846249 MB-527
GSM846250 MB-528
GSM846251 MB-529
This SubSeries is part of SuperSeries:
GSE34356 Clonal Selection Drives Genetic Divergence of Metastatic Medulloblastoma
BioProject PRJNA156527

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34280_RAW.tar 726.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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