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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 01, 2011 |
| Title |
Transgenic Expression of A238L, a Virus Host Modification Protein, in mouse T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The A238L gene has been selectively expressed in mouse T lymphocytes using tissue specific promoter, enhancer and locus control region sequences for CD2. The resulting two independently derived transgenic mice expressed the transgene and developed a metastatic, angiogenic and transplantable CD4+CD8+ CD69- lymphoma. The absence of CD69 from the tumour cells suggests that they were derived from T-cells at a stage prior to positive selection. In contrast, transgenic mice similarly expressing a mutant A238L, mutA238L, solely inhibiting transcription mediated by NFkappaB, were indistinguishable from wild type mice. Expression of Rag 1, Rag2, TCRbeta V8.2, CD25, FoxP3, Bcl3, Bcl2 l14, Myc, IL-2, NFAT1, Itk, by purified CD4+CD8+ CD69- thymocytes from A238L transgenic mice was consistent with the phenotype. Similarly evaluated expression profiles of CD4+CD8+ CD69- thymocytes from the mutant A238L transgenic mice were comparable to those of wild type mice.
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| Overall design |
Purified CD4+CD8+CD69- thymocytes were prepared for wild-type control FVB/N mice, as well as from the FVB/N transgenic mice expressing the A238L and the mutated A238L, mutA238L. Comparisons were performed between the two transgenic mice lines and the control mice.
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| Contributor(s) |
Almeida SC, de Oliveira VL, Bofill M, Parkhouse RM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 30, 2011 |
| Last update date |
May 04, 2018 |
| Contact name |
Sílvia Cristina Paiva Almeida |
| E-mail(s) |
scpalmeida@gmail.com
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| Phone |
00351933287214
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| Organization name |
Instituto Gulbenkian de Ciência
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| Department |
Infection and Immunity
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| Street address |
Rua da Quinta Grande, n 6
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| City |
Oeiras |
| ZIP/Postal code |
2780-156 Oeiras |
| Country |
Portugal |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (3) |
| GSM840978 |
mouse_thymocytes_purified_Wt |
| GSM840979 |
mouse_CD4+CD8+CD69-_thymocytes_purified_A238L |
| GSM840980 |
mouse_CD4+CD8+CD69-_thymocytes_purified_mutA238L |
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| Relations |
| BioProject |
PRJNA149917 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34048_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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