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Series GSE33791 Query DataSets for GSE33791
Status Public on Dec 19, 2011
Title The SNF2-like helicase HELLS mediates E2F3-dependent transcription and cellular transformation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The activating E2F-transcription factors are best known for their dependence on the Retinoblastoma protein and their role in cellular proliferation. E2F3 is uniquely amplified in specific human tumours where its expression is inversely correlated with the survival of patients. Here, E2F3 interaction partners were identified by mass spectrometric analysis. We show that the SNF2-like HELLS interacts with E2F3 in vivo and cooperates with its oncogenic functions. Depletion of HELLS severely perturbs the induction of E2F-target genes, hinders cell cycle re-entry and growth. Using chromatin immmunoprecipitation coupled to sequencing we identified genome-wide targets of HELLS and E2F3. Our analysis revealed that HELLS binds near promoters of active genes, including the trithorax-related MLL1, and co-regulates E2F3-dependent genes. Our analysis is the first to link HELLS with E2F-controlled processes that are critical to establish a proliferative tumour circuitry. Strikingly, just as E2F3, HELLS is overexpressed in human tumours including prostate cancer, indicating that either factor may contribute to the malignant progression of tumours. Our work reveals that HELLS is important for E2F3 in tumour cell proliferation.
Overall design Examination of E2F3, Hells, and H3K27me3 in 2 cell types.
Contributor(s) von Eyss B, Maaskola J, Memczak S, Möllmann K, Schuetz A, Loddenkemper C, Tanh M, Otto A, Muegge K, Heinemann U, Rajewsky N, Ziebold U
Citation(s) 22157815
Submission date Nov 17, 2011
Last update date May 15, 2019
Contact name Jonas Maaskola
Organization name Max-Delbrueck Centrum Berlin-Buch
Department Systems Biology of Gene Regulatory Elements
Lab Rajewsky
Street address Robert-Roessle Str. 10
City Berlin
ZIP/Postal code 13125
Country Germany
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM835826 WT E2F3
GSM835827 KO E2F3
SRA SRP009380
BioProject PRJNA148057

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Supplementary file Size Download File type/resource
GSE33791_RAW.tar 296.3 Mb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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