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Series GSE33633 Query DataSets for GSE33633
Status Public on Jun 04, 2013
Title Epigenetic changes due to histone eviction induced by anthracycline doxorubicin and aclarubicin
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary One major class of anti-cancer drugs targets topoisomerase II to induce DNA double-strand breaks and cell death of fast growing cells. Here, we compare three members of this class - the antracyclines doxorubicin and aclarubicin, and a chemically unrelated compound, etoposide. Aclarubicin does not induce DNA breaks. We define a new activity for the antracyclines: unsupported histone eviction from ┬┤open┬┤ or loosely packed chromosomal areas reflecting exon and promoter regions.
 
Overall design Comparison of histone H3K4me3 of cells post topoisomerase II inhibitors treatment to un-treated ones by ChIP-seq.
Comparison of phosphorylated histone H2AX of cells post topoisomerase II inhibitors doxorubicin and etoposide treatment to un-treated ones by ChIP-seq.
 
Contributor(s) Zwart W, Pang B, Velds A, Neefjes J
Citation(s) 23715267
Submission date Nov 11, 2011
Last update date May 15, 2019
Contact name Baoxu Pang
Organization name Stanford University
Department Genetics
Lab Michael Snyder
Street address 3165 Porter Drive
City Palo Alto
State/province CALIFORNIA
ZIP/Postal code 94304
Country USA
 
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (3)
GSM831874 MelJuSo un-treated H3K4me3 ChIP-seq
GSM831875 MelJuSo doxorubicin H3K4me3 ChIP-seq
GSM831876 MelJuSo etoposide H3K4me3 ChIP-seq
This SubSeries is part of SuperSeries:
GSE33634 Topoisomerase II inhibitors and histone eviction
Relations
SRA SRP009323
BioProject PRJNA154319

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Supplementary file Size Download File type/resource
GSE33633_RAW.tar 1.6 Gb (http)(custom) TAR (of BAM, BED)
GSE33633_rep_1_H3K27me3_cluster.txt.gz 3.7 Mb (ftp)(http) TXT
GSE33633_rep_1_H3K4me3_cluster.txt.gz 3.8 Mb (ftp)(http) TXT
GSE33633_rep_2_H3K27me3_cluster.txt.gz 4.1 Mb (ftp)(http) TXT
GSE33633_rep_2_H3K4me3_cluster.txt.gz 3.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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