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Series GSE3358 Query DataSets for GSE3358
Status Public on Sep 24, 2005
Title DNA damage responses and Mec1p
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Eukaryotic cells respond to DNA damage by arresting the cell cycle and modulating gene expression to ensure efficient DNA repair. The human ATR kinase and its homolog in yeast, MEC1, play central roles in transducing the damage signal. To characterize the role of the Mec1 pathway in modulating the cellular response to DNA damage, we used DNA microarrays to observe genomic expression in Saccharomyces cerevisiae responding to two different DNA-damaging agents. We compared the genome-wide expression patterns of wild-type cells and mutants defective in Mec1 signaling, including mec1, dun1, and crt1 mutants, under normal growth conditions and in response to the methylating-agent methylmethane sulfonate (MMS) and ionizing radiation. Here, we present a comparative analysis of wild-type and mutant cells responding to these DNA-damaging agents, and identify specific features of the gene expression responses that are dependent on the Mec1 pathway. Among the hundreds of genes whose expression was affected by Mec1p, one set of genes appears to represent an MEC1-dependent expression signature of DNA damage. Other aspects of the genomic responses were independent of Mec1p, and likely independent of DNA damage, suggesting the pleiotropic effects of MMS and ionizing radiation. The complete data set as well as supplemental materials is available at http://www-genome.stanford.edu/mec1
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Keywords: Logical Set
 
Overall design Using regression correlation
Web link http://genome-www.stanford.edu/Mec1/
 
Contributor(s) Gasch A
Citation(s) 11598186
Submission date Sep 23, 2005
Last update date Jan 18, 2013
Organization Stanford Microarray Database (SMD)
E-mail(s) array@genome.stanford.edu
Phone 650-498-6012
URL http://genome-www5.stanford.edu/
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (6)
GPL51 y11
GPL55 y15
GPL56 y16
Samples (53)
GSM75932 mec1_plus_gamma_5_min
GSM75933 dun1- + 0.02% MMS - 90 min
GSM75934 DES460 (wt) - mock irradiation - 60 min
Relations
BioProject PRJNA93483

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