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Series GSE33575 Query DataSets for GSE33575
Status Public on Nov 30, 2011
Title Hepatic gene expression profiles after long-term high-fat diet feeding in wild type and Nrf2 knock-out mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary To assess the role of the transcription factor NFE2 related factor 2 like 2 ( Nrf2) in the development of high-fat diet (HFD)-induced obesity and non-alcoholic HFD-induced fatty liver disease, 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) C57BL6J male mice (obtained from Riken BRC, Tsukuba, Japan and originally developed by Prof. M. Yamamoto) were fed an HFD (60 kcal % fat) for 180 days. Whole genome microarray expression profiling was performed in pooled liver samples of WT and Nrf2-KO mice to identify genes that are differentially expressed between WT and Nrf2-KO mice under the stress conditions of HFD-induced obesity.
 
Overall design Liver samples were taken from 8 wild type (WT) and 8 Nrf2 knock-out (Nrf2-KO) male mice on high-fat diet (60 kcal % fat) for 180 days. Total RNA was isolated from pooled liver samples from WT or Nrf2-KO mice to produce 4 samples each using the guanidinium thiocyanate method.
 
Contributor(s) Chartoumpekis D, Zaravinos A, Habeos I
Citation(s) 23710285
Submission date Nov 09, 2011
Last update date Nov 01, 2017
Contact name Dionysios Chartoumpekis
E-mail(s) dchart@upatras.gr
Organization name Lausanne University Hospital CHUV
Department Service of Endocrinology Diabetes and Metabolism
Street address Ave de la Sallaz 8
City Lausanne
ZIP/Postal code 1011
Country Switzerland
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (8)
GSM830131 Liver wild type High Fat Diet, replicate 1
GSM830132 Liver wild type High Fat Diet, replicate 2
GSM830133 Liver wild type High Fat Diet, replicate 3
Relations
BioProject PRJNA148571

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33575_RAW.tar 71.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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