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Series GSE33298 Query DataSets for GSE33298
Status Public on Oct 17, 2012
Title Gene expression analysis of human iPSC generated from pathogenic LRRK2 (G2019S) mutation bearing patients
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Genetic mutations on leucine-rich repeat kinase 2 (LRRK2) have been associated with an increased risk of Parkinson's disease. The Gly2019Ser (G2019S) mutation on LRRK2 gene is a relatively common cause of familial Parkinson's disease in Caucasian population. In this study, we generated human induced pluripotent stem cell (iPSC) lines from LRRK2 (G2019S) bearing patient fibroblasts by cell reprogramming.
We performed global gene expression profiling of LRRK2 (G2019S) heterozygous and homozygous patient iPSC lines, and the corresponding fibroblast lines they originated from. An age-matched wildtype human fibroblast line and H1 human embryonic stem cell (ESC) line were used as controls.
Overall design Microarray gene expression profiling was done to: (1) Compare global gene expression differences between wildtype fibroblasts and fibroblasts from patients bearing homozygous and heterozygous LRRK2 (G2019S) mutation; (2) Compare global gene expression differences between wildtype iPSC and iPSC generated from LRRK2 (G2019S) homozygous and heterozygous patients; (3) Check that all iPSC generated from wildtype and patients fibroblasts are in fact similar to human pluripotent ESC.
Contributor(s) Liu G, Yi F, Izpisua Belmonte J
Citation(s) 23075850
Submission date Oct 27, 2011
Last update date Dec 06, 2018
Contact name Fei Yi
Organization name Salk Institute
Street address 10010 North Torrey Pines Road
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (14)
GSM823565 Wildtype fibroblast sample 1
GSM823566 Wildtype fibroblast sample 2
GSM823567 LRRK2 (G2019S) homozygous fibroblast sample 1
BioProject PRJNA149275

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33298_RAW.tar 28.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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