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Series GSE33058 Query DataSets for GSE33058
Status Public on Jan 31, 2012
Title Transcriptional profiling of disease progression in bovine tuberculosis and the identification of potential diagnostic biomarkers
Organism Mus musculus
Experiment type Expression profiling by array
Summary The definition of biomarkers correlating with disease progression could have impact on the rational design of novel diagnostic approaches for bovine tuberculosis in cattle. We have used the advantages of the murine model to identify promising candidates in the host transcriptome post-infection. RNA from BALB/c splenocytes and lung cells after aerogenic Mycobacterium bovis infection were with high-density microarrays to defining biomarkers of disease progression. In antigen-stimulated splenocytes we found statically significant modulation of 1109 genes early after infection and 1134 at later time-point post-infection. 618 of these genes were modulated at both time points. In the lung 282 genes were significantly modulated post-infection. Amongst the most strongly up-regulated genes were granzyme A in spleen, and cxcl9, granzyme B, interleukin-22, interluekin-17A receptor, and ccr6 in lungs. The expression of 20 of the most up-regulated genes identified in the murine studies were evaluated using PBMC from uninfected and naturally infected cattle. We could show that the expression of cxcl9, granzyme A and interleukin-22 following in vitro stimulation with PPD was significantly increased in infected cows compared to naïve animals. Thus, we have demonstrated that murine transcriptome analysis can be used to predict responses in cattle. In addition, we have prioritised CXCL9, GnzA and IL-22 as potential additional readout systems for the ante-mortem diagnosis of bovine tuberculosis.
Overall design Two groups of 5 BALB/c mice each were infecetd with approx 600 CFU M. bovis via the intranasal route. At days 3 and 14 post challenge five mice per group were euthanized and spleens and lungs harvested. One group of 5 mice were used as a control. Their splenocytes and lung cells were stimulated in vitro for 3 days with M7 protein pool. Antigen cell culture stimulations in mice were performed using an equal pool of seven secreted, immunogenic recombinant mycobacterial proteins (Rv1886c, Rv3019c, Rv3763, Rv3804c, Rv0251, Rv0287 and Rv0288) common to M. bovis and BCG, referred here as M7 protein cocktail. Each protein was used at final concentration of 2μg/ml in 3-day culture.
Contributor(s) Aranday-Cortes E, Hogarth PJ, Kaveh D, Vordermeier M
Citation(s) 22359547
Submission date Oct 18, 2011
Last update date Jan 12, 2017
Contact name ELIHU Aranday-Cortes
Organization name Central Veterinary Laboratories Agency-Weybridge
Department SEB 4
Lab TB Research Group
Street address Woodham Lane, New Haw
State/province SURREY
ZIP/Postal code KT153NB
Country United Kingdom
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (25)
GSM818911 InvitroLung_UU_1
GSM818912 InvitroLung_UU_2
GSM818913 InvitroLung_UU_3
BioProject PRJNA149543

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Supplementary file Size Download File type/resource
GSE33058_RAW.tar 364.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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