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| Status |
Public on Oct 01, 2012 |
| Title |
Phevalin (aureusimine B) production by Staphylococcus aureus biofilm and impacts on human keratinocyte gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Staphylococcus aureus produces the cyclic dipeptides tyrvalin and phevalin (aureusimine A and B, respectively).
A previous study reported that S. aureus mutants not capable of producing these compounds were less virulent in vivo through the deranged regulation of virulence factor genes. These findings, however, have been questioned as an unknown mutation in an operon that regulates virulence was discovered in the mutant strain.
Here, we report that S. aureus biofilms produce greater amounts of phevalin than their planktonic counterparts. When administered to human keratinocytes, phevalin had no substantial effect on gene expression. Phevalin had no obvious impact on the extracellular metabolome of S. aureus.
However, conditioned medium from S. aureus spiked with phevalin resulted in significant differences in keratinocyte gene expression compared to conditioned medium alone.
A role for phevalin in manipulating host responses is apparent. Additionally, phevalin is a potential biomarker and/or therapeutic target for chronic, biofilm-based infections.
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| Overall design |
Secreted factors from S. aureus biofilm and planktonic cultures with equivalent population sizes were placed in contact with human HaCaT keratinocytes for 4 hours. Keratinocytes were grown to ~90% confluency.
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| Contributor(s) |
Secor PR, Jennings LK, James GA, Kirker KR, McInnerney K, Gerlach R, Livinghouse T, Hilmer JK, Bothner B, Fleckman P, Olerud JE, Stewart PS |
| Citation(s) |
22808288 |
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| Submission date |
Oct 12, 2011 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Kate McInnerney |
| E-mail(s) |
kmcinnerney@montana.edu
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| Phone |
406-994-5666
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| Fax |
406-994-4926
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| URL |
http://cores.montana.edu
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| Organization name |
Montana State University
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| Department |
Microbiology
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| Lab |
Functional Genomics Facility
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| Street address |
109 Lewis Hall
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| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (18)
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| GSM815112 |
0.1%DMSO in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 1 |
| GSM815113 |
0.1%DMSO in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 2 |
| GSM815114 |
0.1%DMSO in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 3 |
| GSM815115 |
1 uM phevalin (0.1%DMSO) in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 1 |
| GSM815116 |
1 uM phevalin (0.1%DMSO) in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 2 |
| GSM815117 |
1 uM phevalin (0.1%DMSO) in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 3 |
| GSM815118 |
10 uM phevalin (0.1%DMSO) in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 1 |
| GSM815119 |
10 uM phevalin (0.1%DMSO) in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 2 |
| GSM815120 |
10 uM phevalin (0.1%DMSO) in EpiLife growth medium supplemented with human keratinocyte growth supplement, replicate 3 |
| GSM815121 |
HaCaT cells treated with S.aureus planktonic-conditioned media from cultures exposed to phevalin, replicate 1 |
| GSM815122 |
HaCaT cells treated with S.aureus planktonic-conditioned media from cultures exposed to phevalin, replicate 2 |
| GSM815123 |
HaCaT cells treated with S.aureus planktonic-conditioned media from cultures exposed to phevalin, replicate 3 |
| GSM815124 |
HaCaT cells treated with S.aureus biofilm-conditioned media from cultures spiked with DMSO control, replicate 1 |
| GSM815125 |
HaCaT cells treated with S.aureus biofilm-conditioned media from cultures spiked with DMSO control, replicate 2 |
| GSM815126 |
HaCaT cells treated with S.aureus biofilm-conditioned media from cultures spiked with DMSO control, replicate 3 |
| GSM815127 |
HaCaT cells treated with S.aureus planktonic-conditioned media from cultures spiked with DMSO control, replicate 1 |
| GSM815128 |
HaCaT cells treated with S.aureus planktonic-conditioned media from cultures spiked with DMSO control, replicate 2 |
| GSM815129 |
HaCaT cells treated with S.aureus planktonic-conditioned media from cultures spiked with DMSO control, replicate 3 |
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| Relations |
| BioProject |
PRJNA146481 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32920_MAS5_matrix.txt.gz |
3.2 Mb |
(ftp)(http) |
TXT |
| GSE32920_RAW.tar |
35.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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