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Series GSE32709 Query DataSets for GSE32709
Status Public on Apr 10, 2012
Title DNA methylation regulates lineage-specifying genes in the human vascular system [expression array].
Organism Homo sapiens
Experiment type Expression profiling by array
Summary During embryonic development, the lymphatic system emerges by transdifferentiation from the cardinal vein. Although lymphatic and blood vasculature share a close molecular and developmental relationship, they display distinct features and functions. However, even after terminal differentiation, transitions between the two endothelial cell types have been reported. Since changes in phenotypic plasticity and cellular differentiation processes frequently involve epigenetic mechanisms, we wondered whether DNA methylation might play a role in regulating cell type-specific expression in endothelial cells. By analyzing global gene expression and methylation patterns of primary human dermal lymphatic and blood endothelial cells, we identified a highly significant set of genes, which were differentially methylated and expressed. Pathway analyses of the differentially methylated and upregulated genes in lymphatic endothelial cells revealed involvement in developmental and transdifferentiation processes. We further identified a set of novel genes, which might be implicated in regulating BEC-LEC plasticity and could serve as therapeutic targets and/or biomarkers in vascular diseases associated with alterations in the endothelial phenotype.
Overall design Expression profile of 10 lymphatic endothelial cells was compared to that of 6 blood endothelial cells, no replicates, no control samples.
Contributor(s) Winnefeld M, Brönneke S, Hagemann S
Citation(s) 22434260
Submission date Oct 10, 2011
Last update date Feb 22, 2018
Contact name Silvia Rueberg
Organization name Miltenyi Biotec GmbH
Department Genomic Services Department
Street address Friedrich-Ebert-Str. 68
City Bergisch Gladbach
ZIP/Postal code 51429
Country Germany
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (16)
GSM812754 5112 L
GSM812755 3222 L
GSM812756 20173 L
This SubSeries is part of SuperSeries:
GSE34487 DNA methylation regulates lineage-specifying genes in the human vascular system.
BioProject PRJNA155511

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32709_RAW.tar 152.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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