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Series GSE32690 Query DataSets for GSE32690
Status Public on Dec 21, 2013
Title IFN-γ-induced iNOS expression in regulatory macrophages prolongs allograft graft survival in fully immunocompetent recipients.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse monocytes exposed to M-CSF and IFN-γ were driven to a suppressor phenotype over a seven day culture period. The resulting regulatory macrophages (M regs) expressed markers distinguishing them from M0-, M1-, and M2-polarised macrophages and monocyte-derived DCs. M regs completely suppressed polyclonal T cell proliferation through an inducibile nitric oxide synthase (iNOS)-dependent mechanism. Additionally, M regs were found to eliminate cocultured T cells in an allospecific fashion. In a heterotopic heart transplant model, a single intravenous administration of 5x10^6 donor-strain M regs prior to transplantation significantly prolonged allograft survival in fully immunocompetent recipients using both the stringent C3H-to-BALB/c and C57BL/6-to-BALB/c strain combinations; this graft-protective effect was alloantigen-specific. M regs from Nos2-deficient mice did not prolong allograft survival, proving that M reg function in vivo is iNOS-mediated and dependent on living cells. Co-treatment with 1 mg/kg/day rapamycin for 10 days post-transplant markedly enhanced the effect of M regs. In untransplanted C57BL/6 recipients, M regs of BALB/c origin were detectable for up to 2 weeks post-infusion. It is concluded that mouse M regs represent a novel, phenotypically distinct subset of tolerogenic macrophages, which resemble human M regs in their derivation, phenotype and in vitro functions.
Overall design The dataset comprises 36 samples divided into twelve sample groups each representing a certain monocyte/macrophage subtype
Contributor(s) Riquelme P, Tomiuk S, Kammler A, Fändrich F, Schlitt HJ, Geissler EK, Hutchinson JA
Citation(s) 22929659
Submission date Oct 07, 2011
Last update date Jan 12, 2017
Contact name Stefan Tomiuk
Organization name Miltenyi Biotec GmbH
Department Bioinformatics
Street address Friedrich-Ebert-Str. 68
City Bergisch-Gladbach
ZIP/Postal code 51429
Country Germany
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (36)
GSM812301 Mo-DC_1
GSM812302 Mo-DC_2
GSM812303 Mo-DC_3
BioProject PRJNA146905

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32690_RAW.tar 328.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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