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Series GSE32513 Query DataSets for GSE32513
Status Public on Jul 11, 2012
Title Identification of the core gene-regulatory network that governs the dynamic adaptation of intestinal homeostasis during conventionalization in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Molecular adaptation of the intestinal mucosa occurs during microbial conventionalization to maintain a balanced immune response. However, the genetic regulation of such adaptation is obscure. Here, combined analysis of germ free and conventionalized mice revealed that the major molecular adaptations were initiated at day 4 of conventionalization with a strong induction of innate immune functions followed by stimulation of adaptive immune functions. We identified central regulatory genes and reconstructed a common regulatory network that appeared to be sufficient to regulate the dynamic adaptation of the intestinal mucosa to the colonizing microbiota. The majority of the genes within this regulatory network play roles in mucosal inflammatory diseases in mouse and human. We propose that the identified central regulatory network may serve as a genetic signature for control of intestinal homeostasis in healthy mice and may help to unravel the genetic basis of pathway dysregulation in human intestinal inflammatory diseases.
Overall design Expression profiling of jejunum, ileum, and colon tissue from germ-free and colonized mice at day 1,2,4,8,16 and 30.
Contributor(s) El Aidy S, van Baarlen P, Hooiveld GJ, Kleerebezem M
Citation(s) 22617837, 22722618, 23178667
Submission date Sep 30, 2011
Last update date Apr 18, 2017
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (144)
GSM805090 jejunum germ-free t=0d_rep1
GSM805091 jejunum germ-free t=0d_rep2
GSM805092 jejunum germ-free t=0d_rep3
BioProject PRJNA147851

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Supplementary file Size Download File type/resource
GSE32513_RAW.tar 677.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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