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Series GSE32491 Query DataSets for GSE32491
Status Public on Sep 30, 2011
Title Autophagy driven by a master regulator of hematopoiesis
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery function in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy can occur in specialized cellular contexts, and mechanisms underlying cell type-specific autophagy are poorly understood. We demonstrate that the master regulator of hematopoiesis GATA-1 directly activates transcription of genes encoding the essential autophagy component Microtubule Associated Protein 1 Light Chain 3B (LC3B) and its homologs (MAP1LC3A, GABARAP, GABARAPL1, GATE-16). In addition, GATA-1 directly activates genes involved in the biogenesis/function of lysosomes, which mediate autophagic protein turnover. We demonstrate that GATA-1 utilizes the forkhead protein FoxO3 to activate select autophagy genes. GATA-1-dependent LC3B induction is tightly coupled to accumulation of the active form of LC3B and autophagosomes, which mediate mitochondrial clearance as a critical step in erythropoiesis. These results illustrate a novel mechanism by which a master regulator of development establishes a genetic network to instigate cell type-specific autophagy.
Overall design Genome-wide maps of GATA1 factor occupancy in primary human PBMC derived erythroblasts
Contributor(s) Kang Y, Sanalkumar R, O'Geen H, Linnemann AK, Chang C, Bouhassira EE, Farnham PJ, Keles S, Bresnick EH
Citation(s) 22025678, 25115889
Submission date Sep 29, 2011
Last update date May 15, 2019
Contact name Emery H Bresnick
Phone 608-265-6446
Fax 608-262-1257
Organization name University of Wisconsin School of Medicine and Public Health
Department Department of Cell and Regenerative Biology
Lab Bresnick Lab
Street address 1111 Highland Avenue
State/province Wisconsin
ZIP/Postal code 53705
Country USA
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (4)
GSM804013 GATA1 Replicate1
GSM804014 GATA1 Replicate2
GSM804015 Input Replicate1
SRA SRP008518
BioProject PRJNA147759

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32491_RAW.tar 2.5 Gb (http)(custom) TAR (of TXT)
GSE32491_called_peak.txt.gz 370.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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