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Series GSE32414 Query DataSets for GSE32414
Status Public on Dec 31, 2014
Title Hypoxia-induced stem-like cancer cells resistance to VEGFR inhibition
Organism Homo sapiens
Experiment type Expression profiling by array
Genome variation profiling by genome tiling array
Summary Developing strategies to overcome the frequent resistance to VEGF/VEGFR inhibitors in patients is a major challenge in antiangiogenic therapy for cancer. Targeting the VEGF pathway in vivo induces hypoxia and therefore enables to study drug-induced hypoxia in tumors. Hypoxia also occurs in the spontaneous evolution of cancers, particularly around areas of necrosis. Using cell markers such as CD133, CXCR4, or ALDH1, we identified stem-like cells in peri-necrotic areas in human biopsies and xenografts models of renal cancers. These stem-like cells were able in vitro to self-renew, form carcinoma spheres and had a tumorigenic potency, thus showing characteristics of stem-like cancer cells. In xenografted human renal cell carcinoma, we further demonstrated a strong link between necrosis, either spontaneous or treatment-induced, and the presence of CD133/CXCR4 double positive stem-like cells. Hypoxia increased their tumorigenic potency, induced overexpression of metallothionein2A, which we here identified as critical regulator of resistance to VEGFR-inhibition.
Overall design 6 sample were collected and analysed for gene expression: 2 samples are human tumors xenografted into nude mice, 2 samples are human tumor renal kidney spheres cultured under hypoxic conditions and 2 other samples are human tumor renal kidney spheres cultured under normoxic conditions
Two DNA samples were analysed with CGH array : 1 tumor stem cell cultured in normoxic condition and one human renal carcinoma xenografted into nude mice.
Contributor(s) Varna M, Bousquet G, Feugeas J, Gapihan G, Ferreira I, Leboeuf C, Setterblad N, Duval A, Mongiat-Artus P, Bertheau P, Germain S, Janin A
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Submission date Sep 27, 2011
Last update date Jan 23, 2019
Contact name Anne Janin
Phone +33142494012
Organization name University Paris 7, INSERM, APHP
Street address 1 Av C Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platforms (2)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
GPL10123 Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version)
Samples (8)
GSM802240 1_Hyx
GSM802241 5_Hyx
GSM802242 1_N
BioProject PRJNA146383

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32414_RAW.tar 91.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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