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Series GSE32407 Query DataSets for GSE32407
Status Public on Nov 30, 2011
Title A single intradermal injection of IFN-γ induces a psoriasis-like state in both non-lesional psoriatic and healthy skin
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Psoriasis is a chronic, debilitating, immune-mediated inflammatory skin disease. As IFN-γ is involved in many cellular processes, including activation of T cells and dendritic cells (DCs), antigen processing and presentation, cell adhesion and trafficking, and cytokine and chemokine production, IFN-γ-producing Th1 cells were proposed to be integral to the pathogenesis of psoriasis. Recently, IFN-γ was shown to enhance IL-23 and IL-1 production by DCs and subsequently induce Th17 cells, important contributors to the inflammatory cascade in psoriasis lesions. To determine if IFN-γ indeed induces the pathways leading to the development of psoriasis lesions, a single intradermal injection of IFN-γ was administered to an area of clinically normal, non-lesional skin of psoriasis patients and biopsies were collected 24 hours later. Although there were no visible changes in the skin, IFN-γ induced molecular and histological features characteristic of psoriasis lesions. IFN-γ increased a number of differentially expressed genes in the skin, including many chemokines concomitant with an influx of T cells and inflammatory DCs. Furthermore, inflammatory DC products TNF, iNOS, IL-23, and TRAIL were present in IFN-γ-treated skin. Thus, IFN-γ, which is significantly elevated in non-lesional skin compared to healthy skin, appears to be a key pathogenic cytokine that can induce the inflammatory cascade in psoriasis.
Overall design RNA was isolated from whole skin punch biopsies of either healthy or non-lesional psoraisis patients at baseline or 24 hours after placebo or IFN-g injection.
Contributor(s) Suárez-Fariñas M, Johnson-Huang LM, Lowes MA
Citation(s) 22277938
Submission date Sep 27, 2011
Last update date Dec 06, 2018
Contact name Mayte Suarez-Farinas
Organization name Mount SinaiSchool of Medicine
Street address 1425 Madison Ave, L2-70C, Box 1077,
City New York
State/province NY
ZIP/Postal code 10075
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (60)
GSM802135 Healthy-1 baseline
GSM802136 Healthy-1 +placebo
GSM802137 Healthy-1 +IFN-γ
BioProject PRJNA146401

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Supplementary file Size Download File type/resource
GSE32407_RAW.tar 129.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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