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Series GSE32385 Query DataSets for GSE32385
Status Public on Dec 06, 2011
Title Human PC-3 prostate cancer cells: Control (shNTC; non-target shRNA) vs. shETV4 knock down (shETV4)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transcriptional profiling of human PC-3 prostate cancer cells lentivirally infected with non-target control (NTC) short hairpin (sh)RNA comparing with lentivirally shRNA mediated human ETV4 knock-down. Cells were either cultured for 24 hours at 20% oxygen tension or 0.2% oxygen. Goal was to determine (i) genes affected by hypoxia in PC-3 NTC cells and (ii) identification of hypoxically induced genes requiring ETV4 for hypoxic regulation.
Overall design four condition experiment, shNTC at 20% and 0.2% oxygen and shETV4 at 20% and 0.2% oxygen for 24 hours. Biological replicates: 3 for each of the 4 conditions
Contributor(s) Wollenick K, Wenger RH, Stiehl DP
Citation(s) 22075993
Submission date Sep 26, 2011
Last update date Nov 27, 2018
Contact name Daniel Philipp Stiehl
Organization name University of Zurich
Department Physiology
Street address Winterthurerstr. 190
City Z├╝rich
ZIP/Postal code 8057
Country Switzerland
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM801610 ETV4 KD Hypoxia Replicate 1
GSM801611 ETV4 KD Hypoxia Replicate 2
GSM801612 ETV4 KD Hypoxia Replicate 3
BioProject PRJNA147965

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32385_RAW.tar 40.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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