NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE32385 Query DataSets for GSE32385
Status Public on Dec 06, 2011
Title Human PC-3 prostate cancer cells: Control (shNTC; non-target shRNA) vs. shETV4 knock down (shETV4)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transcriptional profiling of human PC-3 prostate cancer cells lentivirally infected with non-target control (NTC) short hairpin (sh)RNA comparing with lentivirally shRNA mediated human ETV4 knock-down. Cells were either cultured for 24 hours at 20% oxygen tension or 0.2% oxygen. Goal was to determine (i) genes affected by hypoxia in PC-3 NTC cells and (ii) identification of hypoxically induced genes requiring ETV4 for hypoxic regulation.
 
Overall design four condition experiment, shNTC at 20% and 0.2% oxygen and shETV4 at 20% and 0.2% oxygen for 24 hours. Biological replicates: 3 for each of the 4 conditions
 
Contributor(s) Wollenick K, Wenger RH, Stiehl DP
Citation(s) 22075993
Submission date Sep 26, 2011
Last update date Nov 27, 2018
Contact name Daniel Philipp Stiehl
E-mail(s) kristin.wollenick@uzh.ch
Organization name University of Zurich
Department Physiology
Street address Winterthurerstr. 190
City Z├╝rich
ZIP/Postal code 8057
Country Switzerland
 
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM801610 ETV4 KD Hypoxia Replicate 1
GSM801611 ETV4 KD Hypoxia Replicate 2
GSM801612 ETV4 KD Hypoxia Replicate 3
Relations
BioProject PRJNA147965

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32385_RAW.tar 40.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap