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Status |
Public on Dec 06, 2011 |
Title |
Human PC-3 prostate cancer cells: Control (shNTC; non-target shRNA) vs. shETV4 knock down (shETV4) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Transcriptional profiling of human PC-3 prostate cancer cells lentivirally infected with non-target control (NTC) short hairpin (sh)RNA comparing with lentivirally shRNA mediated human ETV4 knock-down. Cells were either cultured for 24 hours at 20% oxygen tension or 0.2% oxygen. Goal was to determine (i) genes affected by hypoxia in PC-3 NTC cells and (ii) identification of hypoxically induced genes requiring ETV4 for hypoxic regulation.
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Overall design |
four condition experiment, shNTC at 20% and 0.2% oxygen and shETV4 at 20% and 0.2% oxygen for 24 hours. Biological replicates: 3 for each of the 4 conditions
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Contributor(s) |
Wollenick K, Wenger RH, Stiehl DP |
Citation(s) |
22075993 |
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Submission date |
Sep 26, 2011 |
Last update date |
Nov 27, 2018 |
Contact name |
Daniel Philipp Stiehl |
E-mail(s) |
kristin.wollenick@uzh.ch
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Organization name |
University of Zurich
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Department |
Physiology
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Street address |
Winterthurerstr. 190
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City |
Zürich |
ZIP/Postal code |
8057 |
Country |
Switzerland |
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Platforms (1) |
GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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Samples (6)
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Relations |
BioProject |
PRJNA147965 |