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Series GSE32340 Query DataSets for GSE32340
Status Public on Apr 05, 2012
Title Reorganization of the host epigenome by a viral oncogene
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Adenovirus small e1a causes ~70% reduction in cellular levels of histone H3 lysine 18 acetylation (H3K18ac). It is unclear, however, where this dramatic reduction occurs genome-wide. ChIP-seq revealed that e1a erases 95% of H3K18ac peaks in normal fibroblasts and replaces them with one-third as many at new genomic locations. H3K18ac at promoters and intergenic regions of genes with fibroblast-related functions are relocalized after infection to promoters of highly-induced genes that regulate cell cycling and to new putative enhancers. Strikingly, a significant fraction of the post-infection H3K18ac peaks occurs precisely at regions bound by RB1 in uninfected cells, but not by p107 or p130 without RB1. In contrast, over half of H3K9ac peaks are similarly distributed before and after infection, independently of RB1. The strategic redistribution of H3K18ac by e1a highlights the importance of this modification for transcriptional activation and cellular transformation.
Overall design Examination of two histone acetylations and RB family members binding.
mRNA-Seq RPKM file linked as supplementary file on Series record.
Contributor(s) Roberto F, Su T, Li B, Bonora G, Oberai A, Sasidharan R, Chan Y, Berk AJ, Pellegrini M, Kurdistani SK
Citation(s) 22499665
Submission date Sep 23, 2011
Last update date May 15, 2019
Contact name Siavash K Kurdistani
Organization name UCLA
Department Biological Chemistry
Lab Kurdistani
Street address 615 Charles E Young Dr South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (15)
GSM802399 Mock H3K18ac_ChIP-seq
GSM802400 e1a H3K18ac_ChIP-seq
GSM802401 Mock H3K9ac_ChIP-seq
BioProject PRJNA147251
SRA SRP008554

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32340_RAW.tar 6.0 Gb (http)(custom) TAR (of BED, WIG)
GSE32340_RPKM_mock_e1a_samples.txt.gz 609.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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