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Series GSE32330 Query DataSets for GSE32330
Status Public on Nov 30, 2011
Title Expression data from C/EBP alpha induced transdifferentiation of pre-B cells into macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Earlier work has shown that pre-B cells can be converted into macrophages by the transcription factor C/EBP? at very high frequencies. Using this system we have now performed a systematic analysis of the question whether during transdifferentiation the cells transiently reactivate progenitor restricted genes or even retrodifferentiate. A transcriptome analysis of transdifferentiating cells showed that most genes are continuously up or downregulated, acquiring a macrophage phenotype within 5 days. In addition, we observed the transient reactivation of a subset of immature myeloid markers, as well as low levels of the progenitor markers Kit and Flt3 and a few lineage inappropriate genes. However, we were unable to observe the re-expression of cell surface marker combinations that characterize hematopoietic stem and progenitor cells (HSPCs), including c-Kit and Flt3. This was the case even when C/EBPalpha was activated in pre-B cells under culture conditions that favor HSPC growth or when the transcription factor was activated in a time limited fashion. Together, our findings are consistent with the notion that the conversion from pre-B cells to macrophages is mostly direct and does not involve overt retrodifferentiation.

Our microarray data indicate that most expression changes are direct, and that transdifferentiation does not involve retrodifferentiation. Our data also show that there is a low level transient activation of selected progenitor genes, as well as myeloid precursor genes.
Overall design Primary mouse pre-B cells were infected with an inducible form of C/EBPa and cells induced to transdifferentiate with beta Estradiol (b-Est) harvested at different time points to analyze expression profiles, using Affymetrix 430.2 arrays.
Contributor(s) Di Tullio A, Vu Manh TP, Schubert A, Mansson R, Graf T
Citation(s) 21969581
Submission date Sep 23, 2011
Last update date Feb 11, 2019
Contact name Thien-Phong Vu Manh
Organization name CIML
Street address 163 avenue de Luminy
City marseille
ZIP/Postal code 13288
Country France
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM800839 pre-B_3h_EtOH_rep1
GSM800840 pre-B_3h_EtOH_rep2
GSM800841 pre-B_3h_b-Est_rep1
BioProject PRJNA147261

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Supplementary file Size Download File type/resource
GSE32330_RAW.tar 45.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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