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Series GSE32319 Query DataSets for GSE32319
Status Public on Mar 15, 2012
Title A unique role of Cohesin-SA1 in gene regulation and development [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Vertebrates have two cohesin complexes that consist of Smc1, Smc3, Rad21/Scc1 and either SA1 or SA2, but their functional specificity is unclear. Mouse embryos lacking SA1 show developmental delay and die before birth. Comparison of the genome wide distribution of cohesin in wild-type and SA1-null cells reveals that SA1 is largely responsible for cohesin accumulation at promoters and at sites bound by the insulator protein CTCF. As a consequence, ablation of SA1 alters transcription of genes involved in biological processes related to Cornelia de Lange syndrome (CdLS), a genetic disorder linked to dysfunction of cohesin. We show that the presence of cohesin-SA1 at the promoter of myc and of protocadherin genes positively regulates their expression, a task that cannot be assumed by cohesin-SA2. Cohesin binding pattern along some gene clusters is also affected by the lack of SA1, leading to dysregulation of the genes within. We hypothesize that impaired cohesin-SA1 function in gene expression underlies the molecular etiology of CdLS.
Overall design Examination of genome wide distribution of cohesin subunits in wildtype and SA1-null cells
Contributor(s) Remeseiro S, Cuadrado A, Gomez-Lopez G, Pisano DG, Losada A
Citation(s) 22415368
Submission date Sep 22, 2011
Last update date May 15, 2019
Contact name Silvia Remeseiro
Organization name Spanish National Cancer Research Centre (CNIO)
Lab Ana Losada's lab
Street address Calle Melchor Fernandez Almagro 3
City Madrid
State/province Madrid
ZIP/Postal code 28029
Country Spain
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (11)
GSM800623 Input
GSM800624 WT_SA1
GSM800625 WT_SA2
This SubSeries is part of SuperSeries:
GSE32320 A unique role of Cohesin-SA1 in gene regulation and development
SRA SRP008511
BioProject PRJNA154497

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32319_RAW.tar 1.3 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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