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Series GSE32180 Query DataSets for GSE32180
Status Public on Nov 30, 2011
Title MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcripts.
Overall design Round spermatids were purified from adult mouse testes of indicated Miwi genotypes. Total RNA was extracted and subjected to microarray analysis for gene expression profiling.
Contributor(s) Reuter M, Berninger P, Chuma S, Shah H, Hosokawa M, Funaya C, Antony C, Sachidanandam R, Pillai RS
Citation(s) 22121019
Submission date Sep 16, 2011
Last update date Mar 04, 2019
Contact name Ramesh Pillai
Organization name University of Geneva
Department Department of Molecular Biology
Street address 30, Quai Ernest-Ansermet
City Gneveva
ZIP/Postal code CH-1211
Country Switzerland
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (8)
GSM797647 MIWI +/- biological replicate 1
GSM797648 MIWI -/KI biological replicate 1
GSM797649 MIWI -/- biological replicate 1
This SubSeries is part of SuperSeries:
GSE32183 MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing
BioProject PRJNA154867

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32180_RAW.tar 32.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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