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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 30, 2005 |
| Title |
High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridiz |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We attempted to identify candidate genes that are expressed more highly in the ICM than in TE cells. Mouse ES cells are cultured from the ICM, whereas mouse TS cells are cultured from the TE. Although these cells have been cultured in vitro, they represent the in vitro equivalents of the ICM and TE. Therefore, genes which are expressed more highly in ES than TS in microarray studies were good candidates for genes predominantly expressed in the ICM in blastocysts.
Keywords: strain or line design
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| Overall design |
We attempted to identify candidate genes that are expressed more highly in the ICM than in TE cells. Mouse ES cells are cultured from the ICM, whereas mouse TS cells are cultured from the TE. Although these cells have been cultured in vitro, they represent the in vitro equivalents of the ICM and TE. Therefore, genes which are expressed more highly in ES than TS in microarray studies were good candidates for genes predominantly expressed in the ICM in blastocysts.
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| Contributor(s) |
Yoshikawa T, Piao Y, Zhong J, Matoba R, Carter MG, Wang Y, Goldberg I, Ko MS |
| Citation(s) |
16325481 |
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| Submission date |
Aug 27, 2005 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Minoru S.H. Ko |
| E-mail(s) |
kom@mail.nih.gov
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| Phone |
410-558-8359
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| Organization name |
NIH
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| Department |
National Institute on Aging
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| Lab |
Lab of Genetics
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| Street address |
251 Bayview Blvd, Suite 100, 10C
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL870 |
NIA Mouse 22K Microarray v1.0 (Development 60-mer Oligo) |
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| Samples (6)
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| GSM72258 |
ES cells, sample #1(16011321011187) |
| GSM72259 |
ES cells, sample #2(16011321011188) |
| GSM72260 |
ES cells, sample #3(16011321011189) |
| GSM72261 |
TS cells, sample #1(16011321011190) |
| GSM72262 |
TS cells, sample #2(16011321011191) |
| GSM72263 |
TS cells, sample #3(16011321011192) |
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| Relations |
| BioProject |
PRJNA92887 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE3214_RAW.tar |
145.2 Mb |
(http)(custom) |
TAR (of TIFF, TXT) |
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